Methodology to identify poly-ADP-ribose polymerase 1 (PARP1)–mRNA targets by PAR-CLiP

Manana Mekishvili, Elena Matveeva, Yvonne Fondufe-Mittendorf

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Scopus citations

Abstract

There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA–protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages211-228
Number of pages18
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1608
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

  • Chromatin
  • PAR-CliP
  • PARP1
  • RNA-binding proteins
  • Splicing
  • mRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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