Methodology to identify poly-ADP-ribose polymerase 1 (PARP1)–mRNA targets by PAR-CLiP

Manana Mekishvili; Elena Matveeva; Yvonne Fondufe-Mittendorf

doi:10.1007/978-1-4939-6993-7_15

Methodology to identify poly-ADP-ribose polymerase 1 (PARP1)–mRNA targets by PAR-CLiP

Manana Mekishvili, Elena Matveeva, Yvonne Fondufe-Mittendorf

Molecular and Cellular Biochemistry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA–protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	211-228
Number of pages	18
DOIs	https://doi.org/10.1007/978-1-4939-6993-7_15
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1608
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Chromatin
PAR-CliP
PARP1
RNA-binding proteins
Splicing
mRNA

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-6993-7_15

Cite this

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AU - Fondufe-Mittendorf, Yvonne

PY - 2017

Y1 - 2017

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AB - There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA–protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

KW - Chromatin

KW - PAR-CliP

KW - PARP1

KW - RNA-binding proteins

KW - Splicing

KW - mRNA

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BT - Methods in Molecular Biology

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Methodology to identify poly-ADP-ribose polymerase 1 (PARP1)–mRNA targets by PAR-CLiP

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

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