Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts

H. P. Spielmann, S. S. Sastry, J. E. Hearst

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photo-adducts. With these methods we have synthesized 2-3 μmol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography.

Original languageEnglish
Pages (from-to)4514-4518
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number10
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • General

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