Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants

Zhenghe Li; Paulina Alatriste Gonzalez; Zsuzsanna Sasvari; Terri Goss Kinzy; Peter D. Nagy

doi:10.1016/j.virol.2013.09.012

Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants

Zhenghe Li, Paulina Alatriste Gonzalez, Zsuzsanna Sasvari, Terri Goss Kinzy, Peter D. Nagy

Plant Pathology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Replication of tombusviruses and other plus-strand RNA viruses depends on several host factors that are recruited into viral replicase complexes. Previous studies have shown that eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. In this paper, we show that methylation of eEF1A by the METTL10-like See1p methyltransferase is required for tombusvirus and unrelated nodavirus RNA replication in yeast model host. Similar to the effect of SEE1 deletion, yeast expressing only a mutant form of eEF1A lacking the 4 known lysines subjected to methylation supported reduced TBSV accumulation. We show that the half-life of several viral replication proteins is decreased in see1δ yeast or when a mutated eEF1A was expressed as a sole source for eEF1A. Silencing of the plant ortholog of See1 methyltransferase also decreased tombusvirus RNA accumulation in Nicotiana benthamiana.

Original language	English
Pages (from-to)	43-54
Number of pages	12
Journal	Virology
Volume	448
DOIs	https://doi.org/10.1016/j.virol.2013.09.012
State	Published - Jan 5 2014

Bibliographical note

Funding Information:
The authors thank Drs. D. Barajas and J. Pogany for valuable comments. This research was supported by the National Science Foundation ( MCB-1122039 ), and the Kentucky Science Foundation to PDN .

Funding

The authors thank Drs. D. Barajas and J. Pogany for valuable comments. This research was supported by the National Science Foundation ( MCB-1122039 ), and the Kentucky Science Foundation to PDN .

Funders	Funder number
Kentucky Science & Engineering Foundation
National Science Foundation Arctic Social Science Program	1122039
Directorate for Biological Sciences	1122039
National Institute of General Medical Sciences	R01GM057483

Keywords

Host factor
Methyltransferase
Nodaviruses
Plant
Posttranslational modification
Replication
Tomato bushy stunt virus
Tombusviruses
Translation elongation factor 1A
Virus-host interaction
Yeast

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2013.09.012

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title = "Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants",

abstract = "Replication of tombusviruses and other plus-strand RNA viruses depends on several host factors that are recruited into viral replicase complexes. Previous studies have shown that eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. In this paper, we show that methylation of eEF1A by the METTL10-like See1p methyltransferase is required for tombusvirus and unrelated nodavirus RNA replication in yeast model host. Similar to the effect of SEE1 deletion, yeast expressing only a mutant form of eEF1A lacking the 4 known lysines subjected to methylation supported reduced TBSV accumulation. We show that the half-life of several viral replication proteins is decreased in see1δ yeast or when a mutated eEF1A was expressed as a sole source for eEF1A. Silencing of the plant ortholog of See1 methyltransferase also decreased tombusvirus RNA accumulation in Nicotiana benthamiana.",

keywords = "Host factor, Methyltransferase, Nodaviruses, Plant, Posttranslational modification, Replication, Tomato bushy stunt virus, Tombusviruses, Translation elongation factor 1A, Virus-host interaction, Yeast",

author = "Zhenghe Li and Gonzalez, \{Paulina Alatriste\} and Zsuzsanna Sasvari and Kinzy, \{Terri Goss\} and Nagy, \{Peter D.\}",

note = "Funding Information: The authors thank Drs. D. Barajas and J. Pogany for valuable comments. This research was supported by the National Science Foundation ( MCB-1122039 ), and the Kentucky Science Foundation to PDN .",

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AU - Li, Zhenghe

AU - Gonzalez, Paulina Alatriste

AU - Sasvari, Zsuzsanna

AU - Kinzy, Terri Goss

AU - Nagy, Peter D.

N1 - Funding Information: The authors thank Drs. D. Barajas and J. Pogany for valuable comments. This research was supported by the National Science Foundation ( MCB-1122039 ), and the Kentucky Science Foundation to PDN .

PY - 2014/1/5

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N2 - Replication of tombusviruses and other plus-strand RNA viruses depends on several host factors that are recruited into viral replicase complexes. Previous studies have shown that eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. In this paper, we show that methylation of eEF1A by the METTL10-like See1p methyltransferase is required for tombusvirus and unrelated nodavirus RNA replication in yeast model host. Similar to the effect of SEE1 deletion, yeast expressing only a mutant form of eEF1A lacking the 4 known lysines subjected to methylation supported reduced TBSV accumulation. We show that the half-life of several viral replication proteins is decreased in see1δ yeast or when a mutated eEF1A was expressed as a sole source for eEF1A. Silencing of the plant ortholog of See1 methyltransferase also decreased tombusvirus RNA accumulation in Nicotiana benthamiana.

AB - Replication of tombusviruses and other plus-strand RNA viruses depends on several host factors that are recruited into viral replicase complexes. Previous studies have shown that eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. In this paper, we show that methylation of eEF1A by the METTL10-like See1p methyltransferase is required for tombusvirus and unrelated nodavirus RNA replication in yeast model host. Similar to the effect of SEE1 deletion, yeast expressing only a mutant form of eEF1A lacking the 4 known lysines subjected to methylation supported reduced TBSV accumulation. We show that the half-life of several viral replication proteins is decreased in see1δ yeast or when a mutated eEF1A was expressed as a sole source for eEF1A. Silencing of the plant ortholog of See1 methyltransferase also decreased tombusvirus RNA accumulation in Nicotiana benthamiana.

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KW - Nodaviruses

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KW - Posttranslational modification

KW - Replication

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KW - Translation elongation factor 1A

KW - Virus-host interaction

KW - Yeast

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Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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