TY - JOUR
T1 - Mg-SINE
T2 - A short interspersed nuclear element from the rice blast fungus, Magnaporthe grisea
AU - Kachroo, Pradeep
AU - Leong, Sally A.
AU - Chattoo, Bharat B.
PY - 1995/11/21
Y1 - 1995/11/21
N2 - A short interspersed nuclear element, Mg-SINE, was isolated and characterized from the genome of the rice blast fungus, Magnaporthe grisea. Mg-SINE was isolated as an insertion element within Pot2, an inverted-repeat transposon from M. grisea and shows typical features of a mammalian SINE. Mg- SINE is present as a 0.47-kb interspersed sequence at ≃ 100 copies per haploid genome in both rice and non-rice isolates of M. grisea, indicating a common evolutionary origin. Secondary structure analysis of Mg-SINE revealed a tRNA-related region at the 5' end which folds into a cloverleaf structure. Genomic fusions resulting in chimeric Mg-SINEs (Ch-SINEs) composed of a sequence homologous to Mg-SINE at the 3' end and an unrelated sequence at its 5' end were also isolated, indicating that this and other DNA rearrangements mediated by these elements may have a major effect on the genomic architecture of this fungus.
AB - A short interspersed nuclear element, Mg-SINE, was isolated and characterized from the genome of the rice blast fungus, Magnaporthe grisea. Mg-SINE was isolated as an insertion element within Pot2, an inverted-repeat transposon from M. grisea and shows typical features of a mammalian SINE. Mg- SINE is present as a 0.47-kb interspersed sequence at ≃ 100 copies per haploid genome in both rice and non-rice isolates of M. grisea, indicating a common evolutionary origin. Secondary structure analysis of Mg-SINE revealed a tRNA-related region at the 5' end which folds into a cloverleaf structure. Genomic fusions resulting in chimeric Mg-SINEs (Ch-SINEs) composed of a sequence homologous to Mg-SINE at the 3' end and an unrelated sequence at its 5' end were also isolated, indicating that this and other DNA rearrangements mediated by these elements may have a major effect on the genomic architecture of this fungus.
KW - repeated DNA
KW - transposon
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U2 - 10.1073/pnas.92.24.11125
DO - 10.1073/pnas.92.24.11125
M3 - Article
C2 - 7479950
AN - SCOPUS:0028791673
SN - 0027-8424
VL - 92
SP - 11125
EP - 11129
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 24
ER -