Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum

Robert B. Page; James R. Monaghan; Amy K. Samuels; Jeramiah J. Smith; Christopher K. Beachy; S. Randal Voss

doi:10.1016/j.cbpc.2006.06.003

Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum

Robert B. Page, James R. Monaghan, Amy K. Samuels, Jeramiah J. Smith, Christopher K. Beachy, S. Randal Voss

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Ambystomatid salamanders offer several advantages for endocrine disruption research, including genomic and bioinformatics resources, an accessible laboratory model (Ambystoma mexicanum), and natural lineages that are broadly distributed among North American habitats. We used microarray analysis to measure the relative abundance of transcripts isolated from A. mexicanum epidermis (skin) after exogenous application of thyroid hormone (TH). Only one gene had a > 2-fold change in transcript abundance after 2 days of TH treatment. However, hundreds of genes showed significantly different transcript levels at days 12 and 28 in comparison to day 0. A list of 123 TH-responsive genes was identified using statistical, BLAST, and fold level criteria. Cluster analysis identified two groups of genes with similar transcription patterns: up-regulated versus down-regulated. Most notably, several keratins exhibited dramatic (1000 fold) increases or decreases in transcript abundance. Keratin gene expression changes coincided with morphological remodeling of epithelial tissues. This suggests that keratin loci can be developed as sensitive biomarkers to assay temporal disruptions of larval-to-adult gene expression programs. Our study has identified the first collection of loci that are regulated during TH-induced metamorphosis in a salamander, thus setting the stage for future investigations of TH disruption in the Mexican axolotl and other salamanders of the genus Ambystoma.

Original language	English
Pages (from-to)	15-27
Number of pages	13
Journal	Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/j.cbpc.2006.06.003
State	Published - Feb 2007

Bibliographical note

Funding Information:
We acknowledge the service of Donna Walls in the UK Microarray Facility. This project was made possible by grant numbers 5R24RR016344, 2P20RR016741, and 2P20RR016481-04 from the National Center for Research Resources, a component of the National Institutes of Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Aspects of this project were also made possible by funding from the U.S. National Science Foundation (IOB-0242833; DBI-0443496) and the Kentucky Spinal Cord and Head Injury Research Trust.

Keywords

Ambystoma
Endocrine disruption
Keratin
Metamorphosis
Mexican axolotl
Microarray
RT-PCR
Thyroid hormone

ASJC Scopus subject areas

Aquatic Science
Animal Science and Zoology
Biochemistry
Physiology
Health, Toxicology and Mutagenesis
Toxicology
Cell Biology

Access to Document

10.1016/j.cbpc.2006.06.003

Cite this

Page, R. B., Monaghan, J. R., Samuels, A. K., Smith, J. J., Beachy, C. K., & Voss, S. R. (2007). Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 145(1), 15-27. https://doi.org/10.1016/j.cbpc.2006.06.003

@article{6746879ad83f490aaf39bc85e9bdc3bf,

title = "Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum",

abstract = "Ambystomatid salamanders offer several advantages for endocrine disruption research, including genomic and bioinformatics resources, an accessible laboratory model (Ambystoma mexicanum), and natural lineages that are broadly distributed among North American habitats. We used microarray analysis to measure the relative abundance of transcripts isolated from A. mexicanum epidermis (skin) after exogenous application of thyroid hormone (TH). Only one gene had a > 2-fold change in transcript abundance after 2 days of TH treatment. However, hundreds of genes showed significantly different transcript levels at days 12 and 28 in comparison to day 0. A list of 123 TH-responsive genes was identified using statistical, BLAST, and fold level criteria. Cluster analysis identified two groups of genes with similar transcription patterns: up-regulated versus down-regulated. Most notably, several keratins exhibited dramatic (1000 fold) increases or decreases in transcript abundance. Keratin gene expression changes coincided with morphological remodeling of epithelial tissues. This suggests that keratin loci can be developed as sensitive biomarkers to assay temporal disruptions of larval-to-adult gene expression programs. Our study has identified the first collection of loci that are regulated during TH-induced metamorphosis in a salamander, thus setting the stage for future investigations of TH disruption in the Mexican axolotl and other salamanders of the genus Ambystoma.",

keywords = "Ambystoma, Endocrine disruption, Keratin, Metamorphosis, Mexican axolotl, Microarray, RT-PCR, Thyroid hormone",

author = "Page, {Robert B.} and Monaghan, {James R.} and Samuels, {Amy K.} and Smith, {Jeramiah J.} and Beachy, {Christopher K.} and Voss, {S. Randal}",

note = "Funding Information: We acknowledge the service of Donna Walls in the UK Microarray Facility. This project was made possible by grant numbers 5R24RR016344, 2P20RR016741, and 2P20RR016481-04 from the National Center for Research Resources, a component of the National Institutes of Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Aspects of this project were also made possible by funding from the U.S. National Science Foundation (IOB-0242833; DBI-0443496) and the Kentucky Spinal Cord and Head Injury Research Trust.",

year = "2007",

month = feb,

doi = "10.1016/j.cbpc.2006.06.003",

language = "English",

volume = "145",

pages = "15--27",

number = "1",

}

Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum. / Page, Robert B.; Monaghan, James R.; Samuels, Amy K. et al.
In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 145, No. 1, 02.2007, p. 15-27.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum

AU - Page, Robert B.

AU - Monaghan, James R.

AU - Samuels, Amy K.

AU - Smith, Jeramiah J.

AU - Beachy, Christopher K.

AU - Voss, S. Randal

N1 - Funding Information: We acknowledge the service of Donna Walls in the UK Microarray Facility. This project was made possible by grant numbers 5R24RR016344, 2P20RR016741, and 2P20RR016481-04 from the National Center for Research Resources, a component of the National Institutes of Health. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Aspects of this project were also made possible by funding from the U.S. National Science Foundation (IOB-0242833; DBI-0443496) and the Kentucky Spinal Cord and Head Injury Research Trust.

PY - 2007/2

Y1 - 2007/2

N2 - Ambystomatid salamanders offer several advantages for endocrine disruption research, including genomic and bioinformatics resources, an accessible laboratory model (Ambystoma mexicanum), and natural lineages that are broadly distributed among North American habitats. We used microarray analysis to measure the relative abundance of transcripts isolated from A. mexicanum epidermis (skin) after exogenous application of thyroid hormone (TH). Only one gene had a > 2-fold change in transcript abundance after 2 days of TH treatment. However, hundreds of genes showed significantly different transcript levels at days 12 and 28 in comparison to day 0. A list of 123 TH-responsive genes was identified using statistical, BLAST, and fold level criteria. Cluster analysis identified two groups of genes with similar transcription patterns: up-regulated versus down-regulated. Most notably, several keratins exhibited dramatic (1000 fold) increases or decreases in transcript abundance. Keratin gene expression changes coincided with morphological remodeling of epithelial tissues. This suggests that keratin loci can be developed as sensitive biomarkers to assay temporal disruptions of larval-to-adult gene expression programs. Our study has identified the first collection of loci that are regulated during TH-induced metamorphosis in a salamander, thus setting the stage for future investigations of TH disruption in the Mexican axolotl and other salamanders of the genus Ambystoma.

AB - Ambystomatid salamanders offer several advantages for endocrine disruption research, including genomic and bioinformatics resources, an accessible laboratory model (Ambystoma mexicanum), and natural lineages that are broadly distributed among North American habitats. We used microarray analysis to measure the relative abundance of transcripts isolated from A. mexicanum epidermis (skin) after exogenous application of thyroid hormone (TH). Only one gene had a > 2-fold change in transcript abundance after 2 days of TH treatment. However, hundreds of genes showed significantly different transcript levels at days 12 and 28 in comparison to day 0. A list of 123 TH-responsive genes was identified using statistical, BLAST, and fold level criteria. Cluster analysis identified two groups of genes with similar transcription patterns: up-regulated versus down-regulated. Most notably, several keratins exhibited dramatic (1000 fold) increases or decreases in transcript abundance. Keratin gene expression changes coincided with morphological remodeling of epithelial tissues. This suggests that keratin loci can be developed as sensitive biomarkers to assay temporal disruptions of larval-to-adult gene expression programs. Our study has identified the first collection of loci that are regulated during TH-induced metamorphosis in a salamander, thus setting the stage for future investigations of TH disruption in the Mexican axolotl and other salamanders of the genus Ambystoma.

KW - Ambystoma

KW - Endocrine disruption

KW - Keratin

KW - Metamorphosis

KW - Mexican axolotl

KW - Microarray

KW - RT-PCR

KW - Thyroid hormone

UR - http://www.scopus.com/inward/record.url?scp=33846795806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846795806&partnerID=8YFLogxK

U2 - 10.1016/j.cbpc.2006.06.003

DO - 10.1016/j.cbpc.2006.06.003

M3 - Article

C2 - 16926121

AN - SCOPUS:33846795806

SN - 1532-0456

VL - 145

SP - 15

EP - 27

JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology

IS - 1

ER -

Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this