Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.
|Title of host publication||Plant Transcription Factors|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||23|
|State||Published - 2011|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
M.G. was supported by a Netherlands Genomic Initiative Horizon grant. A.H. was supported by a Technology Top Institute Green Genetics grant. A.S. was supported by NIH P20 RR16481 and NSF EPS-0814194.
© 2011, Springer Science+Business Media, LLC.
- inducible gene expression systems
- relative quantification, quantitative real-time RT-PCR, endogenous reference gene, amplification efficiency
- target gene
- transcription factor
ASJC Scopus subject areas
- Molecular Biology