Microarray-Based Identification of Transcription Factor Target Genes

Maartje Gorte; Anneke Horstman; Robert B. Page; Renze Heidstra; Arnold Stromberg; Kim Boutilier

doi:10.1007/978-1-61779-154-3_7

Microarray-Based Identification of Transcription Factor Target Genes

Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg, Kim Boutilier

Dr. Bing Zhang Department of Statistics

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.

Original language	English
Title of host publication	Plant Transcription Factors
Subtitle of host publication	Methods and Protocols
Pages	119-141
Number of pages	23
DOIs	https://doi.org/10.1007/978-1-61779-154-3_7
State	Published - 2011

Publication series

Name	Methods in Molecular Biology
Volume	754
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
M.G. was supported by a Netherlands Genomic Initiative Horizon grant. A.H. was supported by a Technology Top Institute Green Genetics grant. A.S. was supported by NIH P20 RR16481 and NSF EPS-0814194.

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Keywords

Microarray
cycloheximide
inducible gene expression systems
relative quantification, quantitative real-time RT-PCR, endogenous reference gene, amplification efficiency
target gene
transcription factor

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-61779-154-3_7

Cite this

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AU - Boutilier, Kim

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Microarray-Based Identification of Transcription Factor Target Genes

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

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