Microarray-Based Identification of Transcription Factor Target Genes

Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg, Kim Boutilier

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations


Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.

Original languageEnglish
Title of host publicationPlant Transcription Factors
Subtitle of host publicationMethods and Protocols
Number of pages23
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
M.G. was supported by a Netherlands Genomic Initiative Horizon grant. A.H. was supported by a Technology Top Institute Green Genetics grant. A.S. was supported by NIH P20 RR16481 and NSF EPS-0814194.

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.


  • Microarray
  • cycloheximide
  • inducible gene expression systems
  • relative quantification, quantitative real-time RT-PCR, endogenous reference gene, amplification efficiency
  • target gene
  • transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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