Microbiological characterization in children with aggressive periodontitis

L. M. Shaddox, H. Huang, T. Lin, W. Hou, P. L. Harrison, I. Aukhil, C. B. Walker, V. Klepac-Ceraj, B. J. Paster

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).Abbreviations: healthy site in healthy sibling (HS); healthy site in healthy control child (HC).

Original languageEnglish
Pages (from-to)927-933
Number of pages7
JournalJournal of Dental Research
Volume91
Issue number10
DOIs
StatePublished - Oct 2012

Bibliographical note

Funding Information:
We acknowledge financial support of NIH/NIDCR ( R01DE019456 ).

Keywords

  • Aggregatibacter actinomycetemcomitans
  • HOMIM
  • diagnosis
  • localized aggressive periodontitis
  • microbiology
  • subgingival microbiota

ASJC Scopus subject areas

  • Dentistry (all)

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