Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing β-carbolines and indoles with biological activity

Daiani C. Savi; Khaled A. Shaaban; Nathalia Vargas; Larissa V. Ponomareva; Yvelise M. Possiede; Jon S. Thorson; Chirlei Glienke; Jürgen Rohr

doi:10.1007/s00284-014-0724-3

Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing β-carbolines and indoles with biological activity

Daiani C. Savi, Khaled A. Shaaban, Nathalia Vargas, Larissa V. Ponomareva, Yvelise M. Possiede, Jon S. Thorson, Chirlei Glienke, Jürgen Rohr

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Abstract

Endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. Vochysia divergens is a medicinal plant, common in the ‘‘Pantanal’’ region (Brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. During a screening program, an endophytic strain isolated from the V. divergens, was investigated for its potential to show biological activity. The strain was characterized as Microbispora sp. LGMB259 by spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene. Strain LGMB259 was cultivated in R5A medium producing metabolites with significant antibacterial activity. The strain produced 4 chemically related b-carbolines, and 3 Indoles. Compound 1-vinyl-β-carboline-3-carboxylic acid displayed potent activity against the Gram-positive bacterial strains Micrococcus luteus NRRL B-2618 and Kocuria rosea B-1106, and was highly active against two human cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung carcinoma cell line A549, with IC₅₀ values of 9.45 and 24.67 lM, respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed moderate activity against the yeast Saccharomyces cerevisiae ATCC204508, as well as the phytopathogenic fungi Phyllosticta citricarpa LGMB06 and Colletotrichum gloeosporioides FDC83.

Original language	English
Pages (from-to)	345-354
Number of pages	10
Journal	Current Microbiology
Volume	70
Issue number	3
DOIs	https://doi.org/10.1007/s00284-014-0724-3
State	Published - Nov 11 2014

Bibliographical note

Funding Information:
We thank Dr. Jack Goodman (University of Kentucky) for the HRESIMS measurements and Dr. Francisco André Ossamu Tanaka (NAP/MEPA) for the Scanning Electron Microscope analysis. D. C. S. thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for a short-term scholarship. This work was supported in part by the University of Kentucky College of Pharmacy, the University of Kentucky Markey Cancer Center and the National Center for Advancing Translational Sciences (UL1TR000117). This work was also supported by NIH grants CA 091901 and GM 105977 to J.R., and Fundação Araucária—Apoio ao desenvolvimento científico e tecnológico do Paraná.

Publisher Copyright:
© Springer Science+Business Media New York 2014.

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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@article{07be659fd7ac4eee8b487bbe55d3ee3c,

title = "Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing β-carbolines and indoles with biological activity",

abstract = "Endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. Vochysia divergens is a medicinal plant, common in the {\textquoteleft}{\textquoteleft}Pantanal{\textquoteright}{\textquoteright} region (Brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. During a screening program, an endophytic strain isolated from the V. divergens, was investigated for its potential to show biological activity. The strain was characterized as Microbispora sp. LGMB259 by spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene. Strain LGMB259 was cultivated in R5A medium producing metabolites with significant antibacterial activity. The strain produced 4 chemically related b-carbolines, and 3 Indoles. Compound 1-vinyl-β-carboline-3-carboxylic acid displayed potent activity against the Gram-positive bacterial strains Micrococcus luteus NRRL B-2618 and Kocuria rosea B-1106, and was highly active against two human cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung carcinoma cell line A549, with IC50 values of 9.45 and 24.67 lM, respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed moderate activity against the yeast Saccharomyces cerevisiae ATCC204508, as well as the phytopathogenic fungi Phyllosticta citricarpa LGMB06 and Colletotrichum gloeosporioides FDC83.",

author = "Savi, {Daiani C.} and Shaaban, {Khaled A.} and Nathalia Vargas and Ponomareva, {Larissa V.} and Possiede, {Yvelise M.} and Thorson, {Jon S.} and Chirlei Glienke and J{\"u}rgen Rohr",

note = "Funding Information: We thank Dr. Jack Goodman (University of Kentucky) for the HRESIMS measurements and Dr. Francisco Andr{\'e} Ossamu Tanaka (NAP/MEPA) for the Scanning Electron Microscope analysis. D. C. S. thanks the Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior (CAPES) for a short-term scholarship. This work was supported in part by the University of Kentucky College of Pharmacy, the University of Kentucky Markey Cancer Center and the National Center for Advancing Translational Sciences (UL1TR000117). This work was also supported by NIH grants CA 091901 and GM 105977 to J.R., and Funda{\c c}{\~a}o Arauc{\'a}ria—Apoio ao desenvolvimento cient{\'i}fico e tecnol{\'o}gico do Paran{\'a}. Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2014.",

year = "2014",

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doi = "10.1007/s00284-014-0724-3",

language = "English",

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AU - Savi, Daiani C.

AU - Shaaban, Khaled A.

AU - Vargas, Nathalia

AU - Ponomareva, Larissa V.

AU - Possiede, Yvelise M.

AU - Thorson, Jon S.

AU - Glienke, Chirlei

AU - Rohr, Jürgen

N1 - Funding Information: We thank Dr. Jack Goodman (University of Kentucky) for the HRESIMS measurements and Dr. Francisco André Ossamu Tanaka (NAP/MEPA) for the Scanning Electron Microscope analysis. D. C. S. thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for a short-term scholarship. This work was supported in part by the University of Kentucky College of Pharmacy, the University of Kentucky Markey Cancer Center and the National Center for Advancing Translational Sciences (UL1TR000117). This work was also supported by NIH grants CA 091901 and GM 105977 to J.R., and Fundação Araucária—Apoio ao desenvolvimento científico e tecnológico do Paraná. Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014/11/11

Y1 - 2014/11/11

N2 - Endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. Vochysia divergens is a medicinal plant, common in the ‘‘Pantanal’’ region (Brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. During a screening program, an endophytic strain isolated from the V. divergens, was investigated for its potential to show biological activity. The strain was characterized as Microbispora sp. LGMB259 by spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene. Strain LGMB259 was cultivated in R5A medium producing metabolites with significant antibacterial activity. The strain produced 4 chemically related b-carbolines, and 3 Indoles. Compound 1-vinyl-β-carboline-3-carboxylic acid displayed potent activity against the Gram-positive bacterial strains Micrococcus luteus NRRL B-2618 and Kocuria rosea B-1106, and was highly active against two human cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung carcinoma cell line A549, with IC50 values of 9.45 and 24.67 lM, respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed moderate activity against the yeast Saccharomyces cerevisiae ATCC204508, as well as the phytopathogenic fungi Phyllosticta citricarpa LGMB06 and Colletotrichum gloeosporioides FDC83.

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Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing β-carbolines and indoles with biological activity

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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