Microcystin-LR Removal from Water via Enzymatic Linearization and Ultrafiltration

Abelline Fionah; Cannon Hackett; Hazim Aljewari; Laura Brady; Faisal Alqhtani; Isabel C. Escobar; Audie K. Thompson

doi:10.3390/toxins14040231

Microcystin-LR Removal from Water via Enzymatic Linearization and Ultrafiltration

Abelline Fionah
, Cannon Hackett
, Hazim Aljewari
, Laura Brady
, Faisal Alqhtani
, Isabel C. Escobar
, Audie K. Thompson

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystin and e A and MlrA enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95% purity. Additionally, composite membranes made of 95% polysulfone and 5% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR on polysulfone/SPEEK membranes. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the progress of linearization and removal of MC-LR. Results indicate that the MlrA was successful at linearizing MC-LR. Membrane filtration tests showed rejection of 97% of cyclic MC-LR and virtually all linearized MC-LR, with adsorption to the membranes being the main rejection mechanism. Adsorption/desorption tests indicated that methanol could be used to strip residual MC-LR from membranes to regenerate them. This study demonstrates a novel strategy of remediation of microcystin-tainted water, combining linearization of MC-LR to a low-toxicity byproduct along with removal by membrane filtration.

Original language	English
Article number	231
Journal	Toxins
Volume	14
Issue number	4
DOIs	https://doi.org/10.3390/toxins14040231
State	Published - Apr 2022

Bibliographical note

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Funding

This research was funded by Grant Number P30 GM103450 from the National Institute of General Medical Sciences of the National Institutes of Health (NIH), by the National Science Foundation NSF DGE: NRT 1922694 and NSF HRD 2004710, by the Kentucky IDeA Networks of Biomedical Research Excellence (KY-INBRE) grant P20GM103436, and by by the U.S. Geological Survey under grant agreement No. G16AP00040 and administered by the Arkansas Water Resources Center.

Funders	Funder number
Arkansas Water Resources Center, University of Arkansas
National Institutes of Health (NIH)
Kentucky IDeA Networks of Biomedical Research Excellence	P20GM103436
National Science Foundation Arctic Social Science Program	NRT 1922694, HRD 2004710
National Institute of General Medical Sciences DP2GM119177 Sophie Dumont National Institute of General Medical Sciences	P30GM103450, P20GM103436
U.S. Geological Survey	G16AP00040

Keywords

enzyme
harmful algal blooms
linearization
microcystin
ultrafiltration

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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10.3390/toxins14040231

Cite this

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title = "Microcystin-LR Removal from Water via Enzymatic Linearization and Ultrafiltration",

abstract = "Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystin and e A and MlrA enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95\% purity. Additionally, composite membranes made of 95\% polysulfone and 5\% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR on polysulfone/SPEEK membranes. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the progress of linearization and removal of MC-LR. Results indicate that the MlrA was successful at linearizing MC-LR. Membrane filtration tests showed rejection of 97\% of cyclic MC-LR and virtually all linearized MC-LR, with adsorption to the membranes being the main rejection mechanism. Adsorption/desorption tests indicated that methanol could be used to strip residual MC-LR from membranes to regenerate them. This study demonstrates a novel strategy of remediation of microcystin-tainted water, combining linearization of MC-LR to a low-toxicity byproduct along with removal by membrane filtration.",

keywords = "enzyme, harmful algal blooms, linearization, microcystin, ultrafiltration",

author = "Abelline Fionah and Cannon Hackett and Hazim Aljewari and Laura Brady and Faisal Alqhtani and Escobar, \{Isabel C.\} and Thompson, \{Audie K.\}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/toxins14040231",

language = "English",

volume = "14",

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AU - Fionah, Abelline

AU - Hackett, Cannon

AU - Aljewari, Hazim

AU - Brady, Laura

AU - Alqhtani, Faisal

AU - Escobar, Isabel C.

AU - Thompson, Audie K.

PY - 2022/4

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N2 - Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystin and e A and MlrA enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95% purity. Additionally, composite membranes made of 95% polysulfone and 5% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR on polysulfone/SPEEK membranes. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the progress of linearization and removal of MC-LR. Results indicate that the MlrA was successful at linearizing MC-LR. Membrane filtration tests showed rejection of 97% of cyclic MC-LR and virtually all linearized MC-LR, with adsorption to the membranes being the main rejection mechanism. Adsorption/desorption tests indicated that methanol could be used to strip residual MC-LR from membranes to regenerate them. This study demonstrates a novel strategy of remediation of microcystin-tainted water, combining linearization of MC-LR to a low-toxicity byproduct along with removal by membrane filtration.

AB - Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystin and e A and MlrA enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95% purity. Additionally, composite membranes made of 95% polysulfone and 5% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR on polysulfone/SPEEK membranes. Liquid chromatography-mass spectrometry (LC-MS) was used to characterize the progress of linearization and removal of MC-LR. Results indicate that the MlrA was successful at linearizing MC-LR. Membrane filtration tests showed rejection of 97% of cyclic MC-LR and virtually all linearized MC-LR, with adsorption to the membranes being the main rejection mechanism. Adsorption/desorption tests indicated that methanol could be used to strip residual MC-LR from membranes to regenerate them. This study demonstrates a novel strategy of remediation of microcystin-tainted water, combining linearization of MC-LR to a low-toxicity byproduct along with removal by membrane filtration.

KW - enzyme

KW - harmful algal blooms

KW - linearization

KW - microcystin

KW - ultrafiltration

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Microcystin-LR Removal from Water via Enzymatic Linearization and Ultrafiltration

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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