Objective Malignant transformation of oral premalignant lesions is the key process in the progression to oral squamous cell carcinoma (OSCC). Previously, we identified miR-7 and miR-21 as candidate oncogenes and miR-375 and miR-494 as candidate tumor suppressors in OSCC. We aim to evaluate these microRNAs as biomarkers of malignant transformation in oral premalignant lesions. Study Design Formalin-fixed, paraffin-embedded samples from progressive premalignant lesions and paired sequential OSCC tumors at the same site were obtained from same patients (n = 31). Total RNA was extracted and analyzed for microRNA levels using real-time polymerase chain reaction. Results MiR-375 expression in progressive lesions was clearly lower than in nonprogressive control lesions (average eightfold difference, P = .0004). Furthermore, the expression of miR-375 decreased significantly after the progression from premalignant lesion to OSCC (P < .0001). Receiver operating characteristic curve analysis revealed that miR-375 was able to differentiate between progressive and nonprogressive premalignant lesions (P < .0001). Conclusions MiR-375 downregulation in oral premalignant lesions is associated with a higher risk of malignant transformation.
|Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
|Published - Dec 1 2016
Bibliographical noteFunding Information:
This study was funded by the University of Florida institutional funds, Andrew J. Semesco Foundation, Ocala, Florida, USA; UMM ALQURA University, Saudi Arabia; and Saudi Arabian Cultural Mission to the United States.
© 2016 Elsevier Inc.
ASJC Scopus subject areas
- Oral Surgery
- Pathology and Forensic Medicine
- Dentistry (miscellaneous)
- Radiology Nuclear Medicine and imaging