Microsatellite markers for the wood decay fungus phlebiopsis gigantea

Ai Zhong Liu; Nicklas Samils; Brian Higgins; Jan Stenlid; Bernard Slippers; C. Joseph Nairn; Sarah F. Covert

doi:10.1007/s10592-008-9785-9

Microsatellite markers for the wood decay fungus phlebiopsis gigantea

Ai Zhong Liu, Nicklas Samils, Brian Higgins, Jan Stenlid, Bernard Slippers, C. Joseph Nairn, Sarah F. Covert

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Nine polymorphic microsatellite markers were developed for the wood-decay basidiomycete Phlebiopsis gigantea, which is used commercially as a biocontrol agent for annosum root disease on conifers. Microsatellite sequences were isolated from repeat-enriched genomic libraries. Primers flanking these sequences were screened on P. gigantea isolates from Europe and North America. The number of alleles per locus ranged from 5 to 15, and gene diversity ranged from 0.72 to 0.90. These markers should be useful for studies of P. gigantea natural population structure and for making predictions about the impact of P. gigantea application in conifer forests.

Original language	English
Pages (from-to)	1529-1532
Number of pages	4
Journal	Conservation Genetics
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s10592-008-9785-9
State	Published - Sep 2009

Bibliographical note

Funding Information:
Acknowledgments We thank Dr. Craig Newton (ATG genetics, Vancouver, British Columbia; atggenetics.com) for isolating the clones containing P. gigantea loci 1, 2, 5, 18 and 65, and Drs. Harrington, LaFlamme, Tubby, Niemi and Korhonen for sending us P. gigantea isolates. This work was supported by a grant from Georgia’s Traditional Industries Program for Pulp and Paper (to SFC), a gift from Verdera Oy (to SFC), and a fellowship from The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (to NS and JS).

Keywords

Biocontrol
Biological control
Microsatellites
Phlebiopsis gigantea
Rotstop
Simple sequence repeat

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s10592-008-9785-9

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title = "Microsatellite markers for the wood decay fungus phlebiopsis gigantea",

abstract = "Nine polymorphic microsatellite markers were developed for the wood-decay basidiomycete Phlebiopsis gigantea, which is used commercially as a biocontrol agent for annosum root disease on conifers. Microsatellite sequences were isolated from repeat-enriched genomic libraries. Primers flanking these sequences were screened on P. gigantea isolates from Europe and North America. The number of alleles per locus ranged from 5 to 15, and gene diversity ranged from 0.72 to 0.90. These markers should be useful for studies of P. gigantea natural population structure and for making predictions about the impact of P. gigantea application in conifer forests.",

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note = "Funding Information: Acknowledgments We thank Dr. Craig Newton (ATG genetics, Vancouver, British Columbia; atggenetics.com) for isolating the clones containing P. gigantea loci 1, 2, 5, 18 and 65, and Drs. Harrington, LaFlamme, Tubby, Niemi and Korhonen for sending us P. gigantea isolates. This work was supported by a grant from Georgia{\textquoteright}s Traditional Industries Program for Pulp and Paper (to SFC), a gift from Verdera Oy (to SFC), and a fellowship from The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (to NS and JS).",

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AU - Nairn, C. Joseph

AU - Covert, Sarah F.

N1 - Funding Information: Acknowledgments We thank Dr. Craig Newton (ATG genetics, Vancouver, British Columbia; atggenetics.com) for isolating the clones containing P. gigantea loci 1, 2, 5, 18 and 65, and Drs. Harrington, LaFlamme, Tubby, Niemi and Korhonen for sending us P. gigantea isolates. This work was supported by a grant from Georgia’s Traditional Industries Program for Pulp and Paper (to SFC), a gift from Verdera Oy (to SFC), and a fellowship from The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (to NS and JS).

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Microsatellite markers for the wood decay fungus phlebiopsis gigantea

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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