Nine polymorphic microsatellite markers were developed for the wood-decay basidiomycete Phlebiopsis gigantea, which is used commercially as a biocontrol agent for annosum root disease on conifers. Microsatellite sequences were isolated from repeat-enriched genomic libraries. Primers flanking these sequences were screened on P. gigantea isolates from Europe and North America. The number of alleles per locus ranged from 5 to 15, and gene diversity ranged from 0.72 to 0.90. These markers should be useful for studies of P. gigantea natural population structure and for making predictions about the impact of P. gigantea application in conifer forests.
|Number of pages||4|
|State||Published - Sep 2009|
Bibliographical noteFunding Information:
Acknowledgments We thank Dr. Craig Newton (ATG genetics, Vancouver, British Columbia; atggenetics.com) for isolating the clones containing P. gigantea loci 1, 2, 5, 18 and 65, and Drs. Harrington, LaFlamme, Tubby, Niemi and Korhonen for sending us P. gigantea isolates. This work was supported by a grant from Georgia’s Traditional Industries Program for Pulp and Paper (to SFC), a gift from Verdera Oy (to SFC), and a fellowship from The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (to NS and JS).
- Biological control
- Phlebiopsis gigantea
- Simple sequence repeat
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics