TY - JOUR
T1 - Microscopic Investigations of the Infection Process of Choristoneura Fumiferana Nucleopolyhedrovirus in the Spruce Budworm
AU - Brownwright, A. J.
AU - Barrett, J. W.
AU - Ladd, T. R.
AU - Primavera, M.
AU - Sohi, S. S.
AU - Arif, B. M.
AU - Retnakaran, A.
AU - Palli, S. R.
N1 - Publisher Copyright:
© 1998 Microscopy Society of America.
PY - 1998/7/1
Y1 - 1998/7/1
N2 - Choristoneura fumiferana nucleopolyhedrovirus(CfMNPV) is a baculovirus that infects the spruce budworm, Choristoneura fumiferana(Cf), and a few other related species. To study the infection process of this virus within the spruce budworm, we have constructed a recombinant virus (CfMNPV-GFP) expressing the green fluorescence protein(GFP) under the control of the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Each larva was fed 10,000 CfMNPV-GFP occlusion bodies(OB) applied to diet plugs and various tissues were dissected from the larvae at 24 hr intervals and examined for the presence of the OB and/or GFP using a fluorescence microscope. Larvae were also fixed and embedded for light and electron microscopy. GFP-specific fluorescence was first detected as a few spots at the posterior end of the midgut at 72 hr post feeding(PF). By 96 hr PF, the fluorescence had expanded to include adjacent cells and some hemocytes.
AB - Choristoneura fumiferana nucleopolyhedrovirus(CfMNPV) is a baculovirus that infects the spruce budworm, Choristoneura fumiferana(Cf), and a few other related species. To study the infection process of this virus within the spruce budworm, we have constructed a recombinant virus (CfMNPV-GFP) expressing the green fluorescence protein(GFP) under the control of the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Each larva was fed 10,000 CfMNPV-GFP occlusion bodies(OB) applied to diet plugs and various tissues were dissected from the larvae at 24 hr intervals and examined for the presence of the OB and/or GFP using a fluorescence microscope. Larvae were also fixed and embedded for light and electron microscopy. GFP-specific fluorescence was first detected as a few spots at the posterior end of the midgut at 72 hr post feeding(PF). By 96 hr PF, the fluorescence had expanded to include adjacent cells and some hemocytes.
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U2 - 10.1017/S1431927600025204
DO - 10.1017/S1431927600025204
M3 - Article
AN - SCOPUS:85180337055
SN - 1431-9276
VL - 4
SP - 1016
EP - 1017
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
ER -