Microscopic Investigations of the Infection Process of Choristoneura Fumiferana Nucleopolyhedrovirus in the Spruce Budworm

A. J. Brownwright, J. W. Barrett, T. R. Ladd, M. Primavera, S. S. Sohi, B. M. Arif, A. Retnakaran, S. R. Palli

Research output: Contribution to journalArticlepeer-review

Abstract

Choristoneura fumiferana nucleopolyhedrovirus(CfMNPV) is a baculovirus that infects the spruce budworm, Choristoneura fumiferana(Cf), and a few other related species. To study the infection process of this virus within the spruce budworm, we have constructed a recombinant virus (CfMNPV-GFP) expressing the green fluorescence protein(GFP) under the control of the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Each larva was fed 10,000 CfMNPV-GFP occlusion bodies(OB) applied to diet plugs and various tissues were dissected from the larvae at 24 hr intervals and examined for the presence of the OB and/or GFP using a fluorescence microscope. Larvae were also fixed and embedded for light and electron microscopy. GFP-specific fluorescence was first detected as a few spots at the posterior end of the midgut at 72 hr post feeding(PF). By 96 hr PF, the fluorescence had expanded to include adjacent cells and some hemocytes.

Original languageEnglish
Pages (from-to)1016-1017
Number of pages2
JournalMicroscopy and Microanalysis
Volume4
DOIs
StatePublished - Jul 1 1998

Bibliographical note

Publisher Copyright:
© 1998 Microscopy Society of America.

ASJC Scopus subject areas

  • Instrumentation

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