TY - JOUR
T1 - Microsymbiont discrimination mediated by a host-secreted peptide in Medicago truncatula
AU - Yang, Shengming
AU - Wang, Qi
AU - Fedorova, Elena
AU - Liu, Jinge
AU - Qin, Qiulin
AU - Zheng, Qiaolin
AU - Price, Paul A.
AU - Pan, Huairong
AU - Wang, Dong
AU - Griffitts, Joel S.
AU - Bisseling, Ton
AU - Zhu, Hongyan
PY - 2017/6/27
Y1 - 2017/6/27
N2 - The legume-rhizobial symbiosis results in the formation of root nodules that provide an ecological niche for nitrogen-fixing bacteria. However, plant-bacteria genotypic interactions can lead to wide variation in nitrogen fixation efficiency, and it is not uncommon that a bacterial strain forms functional (Fix+) nodules on one plant genotype but nonfunctional (Fix-) nodules on another. Host genetic control of this specificity is unknown. We herein report the cloning of the Medicago truncatula NFS1 gene that regulates the fixation-level incompatibility with the microsymbiont Sinorhizobium meliloti Rm41. We show that NFS1 encodes a nodulespecific cysteine-rich (NCR) peptide. In contrast to the known role of NCR peptides as effectors of endosymbionts' differentiation to nitrogen-fixing bacteroids, we demonstrate that specific NCRs control discrimination against incompatible microsymbionts. NFS1 provokes bacterial cell death and early nodule senescence in an allele-specific and rhizobial strain-specific manner, and its function is dependent on host genetic background.
AB - The legume-rhizobial symbiosis results in the formation of root nodules that provide an ecological niche for nitrogen-fixing bacteria. However, plant-bacteria genotypic interactions can lead to wide variation in nitrogen fixation efficiency, and it is not uncommon that a bacterial strain forms functional (Fix+) nodules on one plant genotype but nonfunctional (Fix-) nodules on another. Host genetic control of this specificity is unknown. We herein report the cloning of the Medicago truncatula NFS1 gene that regulates the fixation-level incompatibility with the microsymbiont Sinorhizobium meliloti Rm41. We show that NFS1 encodes a nodulespecific cysteine-rich (NCR) peptide. In contrast to the known role of NCR peptides as effectors of endosymbionts' differentiation to nitrogen-fixing bacteroids, we demonstrate that specific NCRs control discrimination against incompatible microsymbionts. NFS1 provokes bacterial cell death and early nodule senescence in an allele-specific and rhizobial strain-specific manner, and its function is dependent on host genetic background.
KW - Legumes
KW - NCR peptides
KW - Nitrogen fixation specificity
KW - Nodulation
KW - Symbiosis persistence
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U2 - 10.1073/pnas.1700460114
DO - 10.1073/pnas.1700460114
M3 - Article
C2 - 28607056
AN - SCOPUS:85021435878
SN - 0027-8424
VL - 114
SP - 6848
EP - 6853
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -