Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Yanyuan Lei, Xun Zhu, Wen Xie, Qingjun Wu, Shaoli Wang, Zhaojiang Guo, Baoyun Xu, Xianchun Li, Xuguo Zhou, Youjun Zhang

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

To investigate the response of Plutella xylostella transcriptome in defending against a Bt toxin, high-throughput RNA-sequencing was carried out to examine Cry1Ac-susceptible and -resistant strains. The comparative analysis indentified over 2900 differentially expressed unigenes (DEUs) between these two strains. Gene Ontology analysis placed these unigenes primarily into cell, cell part, organelle, binding, catalytic, cellular process, metabolic process, and response to stimulus categories. Based on pathway analyses, DEUs were enriched in oxidoreductase activity and membrane lipid metabolic processes, and they were also significantly enriched in pathways related to the metabolic and biosynthesis of secondary metabolites. Most of the unigenes involved in the metabolic pathway were up-regulated in resistant strains. Within the ABC transporter pathway, majority of the down-regulated unigenes belong to ABCC2 and ABCC10, respectively, while up-regulated unigenes were mainly categorized as ABCG2. Furthermore, two aminopeptidases, and four cadherins encoding genes were significantly elevated as well. This study provides a transcriptome foundation for the identification and functional characterization of genes involved in the Bt resistance in an agriculturally important insect pest, P. xylostella.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalGene
Volume533
Issue number1
DOIs
StatePublished - Jan 1 2014

Bibliographical note

Funding Information:
We thank the Beijing Genomics Institute at Shenzhen for the assistance in original data processing and related bioinformatics analysis. This work was supported by the National Science and Technology support task ( 2012BAD19B06 ), the National High Technology Research and Development Program of China (863 Program) ( 2012AA101502 ), the Special Fund for Agro-scientific Research in the Public Interest ( 201103021 ), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables .

Keywords

  • ABC transporters
  • Bt resistance
  • Cry1Ac
  • Plutella xylostella
  • RNA-seq

ASJC Scopus subject areas

  • Genetics

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