miFAST: A novel and rapid microRNA target capture method

Ashley M. Poenitzsch Strong, Scott M. Berry, David J. Beebe, Jian Liang Li, Vladimir S. Spiegelman

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.

Original languageEnglish
Pages (from-to)559-566
Number of pages8
JournalMolecular Carcinogenesis
Issue number4
StatePublished - Apr 2018

Bibliographical note

Funding Information:
The authors thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing microarray analysis facilities and services. This research is supported by NIEHS training grant ES007015 (to A.M.P.S), NIAMS grant AR063361 (to V.S.S), and NCI grant CA191550 (to V.S.S).

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.


  • direct mRNA targets
  • miFAST
  • miRNA characterization
  • microRNAs

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research


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