miFAST: A novel and rapid microRNA target capture method

Ashley M. Poenitzsch Strong; Scott M. Berry; David J. Beebe; Jian Liang Li; Vladimir S. Spiegelman

doi:10.1002/mc.22780

miFAST: A novel and rapid microRNA target capture method

Ashley M. Poenitzsch Strong, Scott M. Berry, David J. Beebe, Jian Liang Li, Vladimir S. Spiegelman

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.

Original language	English
Pages (from-to)	559-566
Number of pages	8
Journal	Molecular Carcinogenesis
Volume	57
Issue number	4
DOIs	https://doi.org/10.1002/mc.22780
State	Published - Apr 2018

Bibliographical note

Funding Information:
The authors thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing microarray analysis facilities and services. This research is supported by NIEHS training grant ES007015 (to A.M.P.S), NIAMS grant AR063361 (to V.S.S), and NCI grant CA191550 (to V.S.S).

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.

Keywords

direct mRNA targets
miFAST
miRNA characterization
microRNAs

ASJC Scopus subject areas

Molecular Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/mc.22780

Cite this

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title = "miFAST: A novel and rapid microRNA target capture method",

abstract = "MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.",

keywords = "direct mRNA targets, miFAST, miRNA characterization, microRNAs",

author = "{Poenitzsch Strong}, {Ashley M.} and Berry, {Scott M.} and Beebe, {David J.} and Li, {Jian Liang} and Spiegelman, {Vladimir S.}",

note = "Funding Information: The authors thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing microarray analysis facilities and services. This research is supported by NIEHS training grant ES007015 (to A.M.P.S), NIAMS grant AR063361 (to V.S.S), and NCI grant CA191550 (to V.S.S). Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",

year = "2018",

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doi = "10.1002/mc.22780",

language = "English",

volume = "57",

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T2 - A novel and rapid microRNA target capture method

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AU - Berry, Scott M.

AU - Beebe, David J.

AU - Li, Jian Liang

AU - Spiegelman, Vladimir S.

N1 - Funding Information: The authors thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing microarray analysis facilities and services. This research is supported by NIEHS training grant ES007015 (to A.M.P.S), NIAMS grant AR063361 (to V.S.S), and NCI grant CA191550 (to V.S.S). Publisher Copyright: © 2018 Wiley Periodicals, Inc.

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AB - MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.

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miFAST: A novel and rapid microRNA target capture method

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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