Abstract
MicroRNAs (miRNAs), small 22-25 nucleotide non-coding RNAs, play important roles in cellular and tumor biology. However, characterizing miRNA function remains challenging due to an abundance of predicted targets and an experimental bottleneck in identifying biologically relevant direct targets. Here, we developed a novel technique (miFAST) to identify direct miRNA target genes. Using miFAST, we confirmed several previously reported miR-340 target genes and identified five additional novel direct miR-340 targets in melanoma cells. This methodology can also be efficiently applied for the global characterization of miRNA targets. Utilizing miFAST to characterize direct miRNA targetomes will further our understanding of miRNA biology and function.
Original language | English |
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Pages (from-to) | 559-566 |
Number of pages | 8 |
Journal | Molecular Carcinogenesis |
Volume | 57 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2018 |
Bibliographical note
Funding Information:The authors thank the University of Wisconsin Biotechnology Center Gene Expression Center for providing microarray analysis facilities and services. This research is supported by NIEHS training grant ES007015 (to A.M.P.S), NIAMS grant AR063361 (to V.S.S), and NCI grant CA191550 (to V.S.S).
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
Keywords
- direct mRNA targets
- miFAST
- miRNA characterization
- microRNAs
ASJC Scopus subject areas
- Molecular Biology
- Cancer Research