Minor alkaloids of tobacco release [3H]dopamine from superfused rat striatal slices

Linda P. Dwoskin; Li Hong Teng; Susan T. Buxton; Alain Ravard; Niranjan Deo; Peter A. Crooks

doi:10.1016/0014-2999(95)00077-X

Minor alkaloids of tobacco release [³H]dopamine from superfused rat striatal slices

Linda P. Dwoskin, Li Hong Teng, Susan T. Buxton, Alain Ravard, Niranjan Deo, Peter A. Crooks

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

In addition to S(-)-nicotine, several minor tobacco alkaloids ((±)-nornicotine, anabaseine, S(-)-anabasine, and S(-)-N-methylanabasine) are present in tobacco smoke. This study demonstrates that these alkaloids increase fractional ³H release in a concentration-dependent manner from rat striatal slices preloaded with [³H]dopamine, with desensitization of this response. The rank order of EC₅₀ values was S(-)-nicotine (3.0 ± 2.2 μM) > (±)-nornicotine (6.7 ± 2.1 μM) > anabaseine (15.4 ± 6.1 μM) = S(-)-N-methylanabasine (16.3 ± 4.7 μM) = S(-)-anabasine (19.3 ± 3.2 μM). The alkaloids did not modulate fractional ³H release evoked by electrical-field depolarization. Thus, minor tobacco alkaloids may contribute to the apparent neuroprotective effects of smoking in neurodegenerative diseases.

Original language	English
Pages (from-to)	195-199
Number of pages	5
Journal	European Journal of Pharmacology
Volume	276
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-2999(95)00077-X
State	Published - Mar 24 1995

Bibliographical note

Funding Information:
This work was supported by a grant from the Tobacco and Health Research Institute, University of Kentucky, Lexington, KY, USA.

Keywords

Anabaseine
Anabasine
Dopamine release
N-Methylanabasine
Nicotine
Nornicotine
Striatum

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0014-2999(95)00077-X

Cite this

@article{9fbf669237cd45de9d2760e75892716e,

title = "Minor alkaloids of tobacco release [3H]dopamine from superfused rat striatal slices",

abstract = "In addition to S(-)-nicotine, several minor tobacco alkaloids ((±)-nornicotine, anabaseine, S(-)-anabasine, and S(-)-N-methylanabasine) are present in tobacco smoke. This study demonstrates that these alkaloids increase fractional 3H release in a concentration-dependent manner from rat striatal slices preloaded with [3H]dopamine, with desensitization of this response. The rank order of EC50 values was S(-)-nicotine (3.0 ± 2.2 μM) > (±)-nornicotine (6.7 ± 2.1 μM) > anabaseine (15.4 ± 6.1 μM) = S(-)-N-methylanabasine (16.3 ± 4.7 μM) = S(-)-anabasine (19.3 ± 3.2 μM). The alkaloids did not modulate fractional 3H release evoked by electrical-field depolarization. Thus, minor tobacco alkaloids may contribute to the apparent neuroprotective effects of smoking in neurodegenerative diseases.",

keywords = "Anabaseine, Anabasine, Dopamine release, N-Methylanabasine, Nicotine, Nornicotine, Striatum",

author = "Dwoskin, {Linda P.} and Teng, {Li Hong} and Buxton, {Susan T.} and Alain Ravard and Niranjan Deo and Crooks, {Peter A.}",

note = "Funding Information: This work was supported by a grant from the Tobacco and Health Research Institute, University of Kentucky, Lexington, KY, USA.",

year = "1995",

month = mar,

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doi = "10.1016/0014-2999(95)00077-X",

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AU - Dwoskin, Linda P.

AU - Teng, Li Hong

AU - Buxton, Susan T.

AU - Ravard, Alain

AU - Deo, Niranjan

AU - Crooks, Peter A.

N1 - Funding Information: This work was supported by a grant from the Tobacco and Health Research Institute, University of Kentucky, Lexington, KY, USA.

PY - 1995/3/24

Y1 - 1995/3/24

N2 - In addition to S(-)-nicotine, several minor tobacco alkaloids ((±)-nornicotine, anabaseine, S(-)-anabasine, and S(-)-N-methylanabasine) are present in tobacco smoke. This study demonstrates that these alkaloids increase fractional 3H release in a concentration-dependent manner from rat striatal slices preloaded with [3H]dopamine, with desensitization of this response. The rank order of EC50 values was S(-)-nicotine (3.0 ± 2.2 μM) > (±)-nornicotine (6.7 ± 2.1 μM) > anabaseine (15.4 ± 6.1 μM) = S(-)-N-methylanabasine (16.3 ± 4.7 μM) = S(-)-anabasine (19.3 ± 3.2 μM). The alkaloids did not modulate fractional 3H release evoked by electrical-field depolarization. Thus, minor tobacco alkaloids may contribute to the apparent neuroprotective effects of smoking in neurodegenerative diseases.

AB - In addition to S(-)-nicotine, several minor tobacco alkaloids ((±)-nornicotine, anabaseine, S(-)-anabasine, and S(-)-N-methylanabasine) are present in tobacco smoke. This study demonstrates that these alkaloids increase fractional 3H release in a concentration-dependent manner from rat striatal slices preloaded with [3H]dopamine, with desensitization of this response. The rank order of EC50 values was S(-)-nicotine (3.0 ± 2.2 μM) > (±)-nornicotine (6.7 ± 2.1 μM) > anabaseine (15.4 ± 6.1 μM) = S(-)-N-methylanabasine (16.3 ± 4.7 μM) = S(-)-anabasine (19.3 ± 3.2 μM). The alkaloids did not modulate fractional 3H release evoked by electrical-field depolarization. Thus, minor tobacco alkaloids may contribute to the apparent neuroprotective effects of smoking in neurodegenerative diseases.

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KW - Nornicotine

KW - Striatum

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