miR-146a, an anti-inflammatory microRNA, is shown to be a negative regulator of adipocyte inflammation. However, the functional contribution of miR-146a in the development of obesity is not defined. In order to determine whether miR-146a influences diet-induced obesity, mice that were either wild type (WT) or miR-146a deficient (KO) were fed with high (60% kcal) fat diet (HFD) for 16 weeks. Deficiency of miR-146a did not influence obesity measured as HFD-induced body weight and fat mass gain, or metabolism of glucose and insulin tolerance. In addition, adipocyte apoptosis, adipose tissue collagen and macrophage accumulation as detected by TUNEL, Picro Sirius and F4/80 immunostaining, respectively, were comparable between the two groups of mice. Although, miR-146a deficiency had no influence on HFD-induced hepatic lipid accumulation, interestingly, it significantly increased obesity-induced inflammatory responses in liver tissue. The present study demonstrates that miR-146a deficiency had no influence on the development of HFD-induced obesity and adipose tissue remodeling, whereas it significantly increased hepatic inflammation in obese mice. This result suggests that miR-146a regulates hepatic inflammation during development of obesity.
|State||Published - Dec 1 2019|
Bibliographical noteFunding Information:
We thank Dr. Ryan Temel and Dr. Lei Cai for their help in liver lipid extraction. We appreciate technical help from Dr. Wendy Katz and Munira Nasser in preparing histological tissue sections. We acknowledge the skilled editorial assistance of Debra Rateri. The study was supported by the National Institutes of Health (Grants P20GM103527, R01HL130086). The content in this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
© 2019, The Author(s).
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