miRNP:mRNA association in polyribosomes in a human neuronal cell line

Peter T. Nelson, Artemis G. Hatzigeorgiou, Zissimos Mourelatos

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that control gene expression by base-pairing with their mRNA targets. miRNAs assemble into ribonucleoprotein complexes termed miRNPs. Animal miRNAs recognize their mRNA targets via partial antisense complementarity and repress mRNA translation at a step after translation initiation. How animal miRNAs recognize their mRNA targets and how they control their translation is unknown. Here we describe that in a human neuronal cell line, the miRNP proteins eIF2C2 (a member of the Argonaute family of proteins), Gemin3, and Gemin4 along with miRNAs cosediment with polyribosomes. Furthermore, we describe a physical association between a let-7b (miRNA)-containing miRNP and its putative human mRNA target in polyribosome-containing fractions. These findings suggest that miRNP proteins may play important roles in target mRNA recognition and translational repression.

Original languageEnglish
Pages (from-to)387-394
Number of pages8
JournalRNA
Volume10
Issue number3
DOIs
StatePublished - Mar 2004

Keywords

  • Argonaute
  • Lin-28
  • Polyribosomes
  • Translation
  • miRNP
  • microRNA

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint

Dive into the research topics of 'miRNP:mRNA association in polyribosomes in a human neuronal cell line'. Together they form a unique fingerprint.

Cite this