Abstract
The mitochondrial genome of the S-type male-sterile cytoplasm of maize appears to be a collection of linear DNA molecules that are maintained by two linear episomal DNA species, S1 (6397 bp) and S2 (5453 bp), recombining with larger circular molecules. The M825 inbred nuclear genotype promotes a high frequency of spontaneous fertile mutants. Where the mutation is maternally inherited, analysis of the mitochondrial DNA reveals that the S1 and S2 episomes and all associated linear molecules have been lost. Integrated copies of the S1 and S2 episomes are retained, though part of one of the two terminal inverted repeats of the integrated S2 sequence has been deleted in all revertants studied. This deletion appears to correlate with the absence of the S2-URF-1 transcript.
| Original language | English |
|---|---|
| Pages (from-to) | 361-368 |
| Number of pages | 8 |
| Journal | Cell |
| Volume | 43 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 1985 |
Bibliographical note
Funding Information:This work was supported in part by NATO grant 263.81 to D. M. L. and D. R. P; C. L. S. is a DeKalb-Pfizer Genetics fellow. We thank Pioneer Hybrid International, DeKalb-Pfizer Genetics, Dr. J. Laughnan, and Dr. S. Gabay-Laughnan for supplying seed; Tony P. Hodge for technical assistance; and Dr. M.-R. Fauron for preparation of the R369 cosmid bank.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology (all)