Modified fenton reaction for trichlorophenol dechlorination by enzymatically generated H2O2 and gluconic acid chelate

Deepak K. Ahuja, Leonidas G. Bachas, D. Bhattacharyya

Research output: Contribution to journalArticlepeer-review

31 Citations (SciVal)


Glucose oxidase is a well-known enzyme that catalyzes the oxidation of β-d-glucose to produce gluconic acid and hydrogen peroxide. Fenton reaction is a powerful oxidation technology used for the oxidation of groundwater pollutants. For the application of Fenton reaction in groundwater remediation, successful operation of Fenton reaction near neutral pH, and on-site generation of both H2O2 and chelate will be beneficial. The focus of this experimental study was to couple the glucose oxidation reaction with chelate-based Fenton reaction. The idea was to use the hydrogen peroxide and chelate gluconic acid generated during glucose oxidation for the dechlorination of 2,4,6-trichlorophenol (TCP) by Fenton reaction. The oxidation of glucose was achieved using the enzyme in free and immobilized forms. The rate of production of hydrogen peroxide was determined for each system, and was used to estimate the time required for complete consumption of glucose during the process, thus avoiding any traces of glucose in the Fenton reaction. In the case of free enzyme reaction, separation of the enzyme was achieved using an ultrafiltration membrane before initiating the Fenton reaction. The oxidation of TCP by Fenton reaction was performed at varying ratios of gluconic acid/Fe, and its effect on the decomposition of TCP and H2O2 was studied. TCP degradation was studied both in terms of parent compound degradation and free chloride generation.

Original languageEnglish
Pages (from-to)2193-2200
Number of pages8
Issue number11
StatePublished - Feb 2007

Bibliographical note

Funding Information:
This research was supported by the NIEHS-SBRP program. Various analytical supports were provided by ERTL (Environmental Research Training Lab) at the University of Kentucky.


  • Glucose oxidase
  • Hydroxyl radical
  • Immobilization
  • Kinetic model

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry (all)
  • Pollution
  • Health, Toxicology and Mutagenesis


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