Modulation of GmFAD3 expression alters abiotic stress responses in soybean

Ajay Kumar Singh; Susheel Kumar Raina; Mahesh Kumar; Lalitkumar Aher; Milind B. Ratnaparkhe; Jagadish Rane; Aardra Kachroo

doi:10.1007/s11103-022-01295-4

Modulation of GmFAD3 expression alters abiotic stress responses in soybean

Ajay Kumar Singh, Susheel Kumar Raina, Mahesh Kumar, Lalitkumar Aher, Milind B. Ratnaparkhe, Jagadish Rane, Aardra Kachroo

Plant Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Key message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Abstract: Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.

Original language	English
Journal	Plant Molecular Biology
DOIs	https://doi.org/10.1007/s11103-022-01295-4
State	Published - Sep 2022

Bibliographical note

Funding Information:
Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research.

Funding Information:
Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research.

Funding Information:
This work was supported by grant from Indian Council of Agricultural Research-National Agriculture Science Fund (ICAR-NASF, Grant No. NASF/GTR-6035/2018–19).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

Bean pod mottle virus
Drought stress
Fatty acid desaturase
Gene silencing
Jasmonic acid
Overexpression
Salinity stress
WRKY transcription factor

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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10.1007/s11103-022-01295-4

Cite this

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title = "Modulation of GmFAD3 expression alters abiotic stress responses in soybean",

abstract = "Key message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Abstract: Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.",

keywords = "Bean pod mottle virus, Drought stress, Fatty acid desaturase, Gene silencing, Jasmonic acid, Overexpression, Salinity stress, WRKY transcription factor",

author = "Singh, {Ajay Kumar} and Raina, {Susheel Kumar} and Mahesh Kumar and Lalitkumar Aher and Ratnaparkhe, {Milind B.} and Jagadish Rane and Aardra Kachroo",

note = "Funding Information: Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research. Funding Information: Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research. Funding Information: This work was supported by grant from Indian Council of Agricultural Research-National Agriculture Science Fund (ICAR-NASF, Grant No. NASF/GTR-6035/2018–19). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature B.V.",

year = "2022",

month = sep,

doi = "10.1007/s11103-022-01295-4",

language = "English",

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T1 - Modulation of GmFAD3 expression alters abiotic stress responses in soybean

AU - Singh, Ajay Kumar

AU - Raina, Susheel Kumar

AU - Kumar, Mahesh

AU - Aher, Lalitkumar

AU - Ratnaparkhe, Milind B.

AU - Rane, Jagadish

AU - Kachroo, Aardra

N1 - Funding Information: Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research. Funding Information: Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research. Funding Information: This work was supported by grant from Indian Council of Agricultural Research-National Agriculture Science Fund (ICAR-NASF, Grant No. NASF/GTR-6035/2018–19). Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Nature B.V.

PY - 2022/9

Y1 - 2022/9

N2 - Key message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Abstract: Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.

AB - Key message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Abstract: Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.

KW - Bean pod mottle virus

KW - Drought stress

KW - Fatty acid desaturase

KW - Gene silencing

KW - Jasmonic acid

KW - Overexpression

KW - Salinity stress

KW - WRKY transcription factor

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SN - 0167-4412

JO - Plant Molecular Biology

JF - Plant Molecular Biology

ER -

Modulation of GmFAD3 expression alters abiotic stress responses in soybean

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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