Modulation of GmFAD3 expression alters abiotic stress responses in soybean

Ajay Kumar Singh, Susheel Kumar Raina, Mahesh Kumar, Lalitkumar Aher, Milind B. Ratnaparkhe, Jagadish Rane, Aardra Kachroo

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Key message: This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Abstract: Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.

Original languageEnglish
Pages (from-to)199-218
Number of pages20
JournalPlant Molecular Biology
Volume110
Issue number1-2
DOIs
StatePublished - Sep 2022

Bibliographical note

Funding Information:
Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research.

Funding Information:
Authors are grateful to Dr. Himanshu Pathak, Director, ICAR-National Institute of Abiotic Stress Management (ICAR-NIASM) and Dr. Kailash Bansal, the Alliance of Bioversity International & International Centre for Tropical Agriculture (CIAT), for critical review and editing the manuscript. We thank ICAR-NASF (Grant No. NASF/GTR-6035/2018-19) for supporting the research. We also thank ICAR-NIASM, Baramati, Pune, India; and Department of Plant pathology, University of Kentucky, USA for the logistic support provided for conducting research.

Funding Information:
This work was supported by grant from Indian Council of Agricultural Research-National Agriculture Science Fund (ICAR-NASF, Grant No. NASF/GTR-6035/2018–19).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

  • Bean pod mottle virus
  • Drought stress
  • Fatty acid desaturase
  • Gene silencing
  • Jasmonic acid
  • Overexpression
  • Salinity stress
  • WRKY transcription factor

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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