Molecular bone morphometry

M. C. Langub, M. C. Faugere, H. H. Malluche

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Quantitative histomorphometric assessment of bone biopsies represents a powerful and informative method for the study of metabolic bone diseases. It is the gold standard against which the noninvasive 'diagnostic' markers of bone metabolism as well as newly available therapeutic modalities are tested. With the rapid progress in technology of molecular biology, identification of systemic and local biomolecules known to regulate bone metabolism can now be achieved. The study of localization, levels of expression, and synthesis of these factors in bone and its microenvironment is possible through applications of in situ hybridization histochemistry (ISHH) and immunohistochemistry (IHC). Application of ISHH allows study of specific mRNA expression. IHC determines the presence and distribution of target protein in cells. These two methodologies provide the link between the cellular processes of mRNA transcription and translation to the working protein. Combining the established bone histomorphometric techniques with ISHH and IHC elevates the study of bone to new heights, i.e., cellular and molecular mechanistic issues can now be studied.

Original languageEnglish
Pages (from-to)629-635
Number of pages7
JournalPediatric Nephrology
Volume14
Issue number7
DOIs
StatePublished - Jul 2000

Keywords

  • Bone
  • Histomorphometry
  • Immunohistochemistry
  • In situ hybridization histochemistry
  • Molecular morphometry

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Nephrology

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