Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars.

Shuyu Liu, Carl A. Griffey, Marla D. Hall, Anne L. McKendry, Jianli Chen, Wynse S. Brooks, Gina Brown-Guedira, David Van Sanford, David G. Schmale

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

In the soft red winter wheat (Triticum aestivum L.) regions of the US, Fusarium head blight (FHB, caused by Fusarium spp.) resistance derived from locally adapted germplasm has been used predominantly. Two soft red winter wheat cultivars, Massey and Ernie, have moderate resistance to FHB. Mapping populations derived from Becker/Massey (B/M) and Ernie/MO 94-317 (E/MO) were evaluated for FHB resistance and other traits in multiple environments. Eight QTL in B/M and five QTL in E/MO were associated with FHB variables including incidence, severity (SEV), index (IND), Fusarium damaged kernels (FDK), deoxynivalenol (DON), and morphological traits flowering time and plant height. Four QTL were common to both populations. Three of them were located at or near known genes: Ppd-D1 on chromosome 2DS, Rht-B1 on 4BS, and Rht-D1 on 4DS. Alleles for dwarf plant height (Rht-B1b and Rht-D1b) and photoperiod insensitivity (Ppd-D1a) had pleiotropic effects in reducing height and increasing FHB susceptibility. The other QTL detected for FHB variables were on 3BL in both populations, 1AS, 1DS, 2BL, and 4DL in B/M, and 5AL (B1) and 6AL in E/MO. The additive effects of FHB variables ranged from 0.4 mg kg(-1) of DON to 6.2 % for greenhouse (GH) SEV in B/M and ranged from 0.3 mg kg(-1) of DON to 8.3 % for GH SEV in E/MO. The 4DS QTL had epistasis with Ppd-D1, Qdon.umc-6AL, and Qht.umc-4BS, and additive × additive × environment interactions with the 4BS QTL for SEV, IND, and FDK in E/MO. Marker-assisted selection might be used to enhance FHB resistance through selection of favorable alleles of significant QTL, taking into account genotypes at Rht-B1b, Rht-D1a and Ppd-D1a.

Original languageEnglish
Pages (from-to)2485-2498
Number of pages14
JournalTheoretical And Applied Genetics
Volume126
Issue number10
DOIs
StatePublished - Oct 2013

Bibliographical note

Funding Information:
The authors thank P. Gundrum, N. McMaster, B. Will, J. Seago, G. Berger, M. Christopher, P. O’Boyle, M. Vaughn, R. Pitman, T. Lewis, D. Donavan, and D. Reaver from Virginia Tech for their technical support. Additional data were kindly provided by D. Tague from University of Missouri–Columbia, N. Mundell from University of Kentucky, J. Smith and M. Xiong from USDA-ARS genotyping center at Raleigh, NC. Funding for this project was provided by the Virginia Small Grains Board, the Virginia Agricultural Council, and the US Department of Agriculture under Agreement No. 59-0790-4-102. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture.

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint

Dive into the research topics of 'Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars.'. Together they form a unique fingerprint.

Cite this