Molecular Characterization of Multidrug-Resistant and Extended-Spectrum β-Lactamases-Producing Salmonella enterica Serovars Enteritidis and Typhimurium Isolated from Raw Meat in Retail Markets

Md Mahfujur Rahman, Hemayet Hossain, Md Shahidur Rahman Chowdhury, Md Mukter Hossain, Asmaa Saleh, Reem Binsuwaidan, Ayman Noreddin, Yosra A. Helmy, Mohamed E. El Zowalaty

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study, a total of 720 samples were collected from retail raw meat from 13 upazilas in Sylhet District, Bangladesh, of which 225 samples were from cattle meat, 210 samples were from goat meat, and 285 samples were from chicken meat. Salmonella enterica serovars Typhimurium and Enteritidis were screened for extended-spectrum β-lactamase (ESBL) genes using multiplex PCR. Among the 720 samples, Salmonella spp. was detected in 28.06% (202 out of 720) of the samples, with S. Enteritidis and S. Typhimurium were identified in 11.53% (83 out of 720) and 12.22% (88 out of 720) of the samples, respectively. It was found that all Salmonella enterica serovars isolated from cattle meat displayed multidrug resistance (MDR) based on antimicrobial susceptibility testing. Notably, a significant proportion of S. Enteritidis isolates and all S. Typhimurium isolates from goat meat demonstrated complete resistance to multiple drugs (ampicillin, cefuroxime, and ceftazidime). Regarding chicken meat, out of 89 isolates encompassing both S. Typhimurium and S. Enteritidis, 57 isolates (64.04%) exhibited MDR. Additionally, blaCTX-M-1 exhibited the highest occurrence at 15.69% for S. Typhimurium and 7.89% for S. Enteritidis in chicken meat. Moreover, blaCTX-M-9 was only detected at 3.92% for S. Enteritidis in chicken meat. Furthermore, blaOXA had the highest prevalence rate of 19.04% for S. Enteritidis and 25.80% for S. Typhimurium in cattle meat, followed by chicken meat. These findings highlight the urgency for monitoring ESBL-producing Salmonella in retail raw meat and the need for strict measure to manage antibiotic use to prevent the spread of multidrug-resistant and ESBL-producing Salmonella strains, thereby protecting humans and reducing public health risks.

Original languageEnglish
Article number586
JournalAntibiotics
Volume13
Issue number7
DOIs
StatePublished - Jul 2024

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Funding

This study was supported in part by funding from the Sylhet Agricultural University Research System (SAURES) under the auspices of the University Grant Commission (UGC) of Bangladesh. The research was supported in part by discretionary funding from Prof. ME El Zowalaty and Dr. Yosra A. Helmy. This research was funded by the Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2024R304), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

FundersFunder number
Sylhet Agricultural University
University Grants Commission
Princess Nourah Bint Abdulrahman UniversityPNURSP2024R304

    Keywords

    • Bangladesh
    • cattle
    • chicken
    • extended-spectrum β-lactamases
    • livestock
    • meat
    • multidrug resistance
    • S. Enteritidis
    • S. Typhimurium

    ASJC Scopus subject areas

    • Microbiology
    • Biochemistry
    • General Pharmacology, Toxicology and Pharmaceutics
    • Microbiology (medical)
    • Infectious Diseases
    • Pharmacology (medical)

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