Molecular cloning and expression of equine interleukin 2

Evie V. Vandergrifft, David W. Horohov

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


We have cloned equine IL-2 cDNA in vitro using the polymerase chain reaction (PCR) and primers based on the human IL-2 sequence. The cloned product appears to contain the entire coding region for equine IL-2 based on homology with other known sequences. When expressed in COS cells, the recombinant product augmented the proliferative response of equine peripheral blood mononuclear cells to concanavalin A, however, it failed to support the continued proliferation of murine CTLL-2 cells. Specific substitutions in those regions associated with p55 and p75 binding appear to account for this species-specifity.

Original languageEnglish
Pages (from-to)395-406
Number of pages12
JournalVeterinary Immunology and Immunopathology
Issue number4
StatePublished - Dec 1993

ASJC Scopus subject areas

  • Immunology
  • General Veterinary


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