Molecular cloning and expression of equine interleukin 2

Evie V. Vandergrifft; David W. Horohov

doi:10.1016/0165-2427(93)90070-K

Molecular cloning and expression of equine interleukin 2

Evie V. Vandergrifft, David W. Horohov

Veterinary Science

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have cloned equine IL-2 cDNA in vitro using the polymerase chain reaction (PCR) and primers based on the human IL-2 sequence. The cloned product appears to contain the entire coding region for equine IL-2 based on homology with other known sequences. When expressed in COS cells, the recombinant product augmented the proliferative response of equine peripheral blood mononuclear cells to concanavalin A, however, it failed to support the continued proliferation of murine CTLL-2 cells. Specific substitutions in those regions associated with p55 and p75 binding appear to account for this species-specifity.

Original language	English
Pages (from-to)	395-406
Number of pages	12
Journal	Veterinary Immunology and Immunopathology
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/0165-2427(93)90070-K
State	Published - Dec 1993

ASJC Scopus subject areas

Immunology
Veterinary (all)

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AB - We have cloned equine IL-2 cDNA in vitro using the polymerase chain reaction (PCR) and primers based on the human IL-2 sequence. The cloned product appears to contain the entire coding region for equine IL-2 based on homology with other known sequences. When expressed in COS cells, the recombinant product augmented the proliferative response of equine peripheral blood mononuclear cells to concanavalin A, however, it failed to support the continued proliferation of murine CTLL-2 cells. Specific substitutions in those regions associated with p55 and p75 binding appear to account for this species-specifity.

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Molecular cloning and expression of equine interleukin 2

Abstract

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