A cDNA clone encoding a 25-kDa protein (25K) was isolated from a cDNA library made from RNA isolated from the adult fat body and ovaries of the locust, Locusta migratoria. The longest open reading frame of this cDNA clone encodes a 225-amino acid polypeptide, the N-terminal end of which was similar to the 21-kDa and 19-kDa juvenile hormone induced proteins identified in the locust hemolymph, but the C-terminal end was different. The C-terminal end of the 25K cDNA contained seven unique repeat elements of 10 amino acids each, most of which are polar residues. Expression of the 25K mRNA was tissue-, development- and sex-specific. A 1.2-kb mRNA was detected using the 25K cDNA as a probe only in the fat body of adult females. The mRNA started to appear at day 4 after the insect molted to the adult and rapidly increased by day 6. The mRNA was absent in the ovarian follicle cells and fat body of adult males. In vitro transcription and translation of the 25K cDNA produced a protein that migrated around 32 kDa on sodium dodecyl sulfate polyacrylamide gels. The 25K cDNA was expressed in a baculovirus expression system and the protein produced also migrated around 32 kDa. (C) 2000 Elsevier Science Ltd.
|Number of pages||9|
|Journal||Insect Biochemistry and Molecular Biology|
|State||Published - Sep 2000|
Bibliographical noteFunding Information:
This research was supported in part by a Natural Sciences and Engineering Research Council of Canada Research Grant, and a Collaborative Research and Development Grant from NSERC and Cyanamid Canada to K.G.D. and by the National Biotechnology Strategy Fund and the Science and Technology Opportunities Fund of the Canadian Forest Service to Biotechnology at GLFC.
- Baculovirus expression system
- Fat body
- Follicle cell
- Repeat element
- Reproductive maturation
ASJC Scopus subject areas
- Molecular Biology
- Insect Science