TY - JOUR
T1 - Molecular cloning of a lobster Gα(q) protein expressed in neurons of olfactory organ and brain
AU - McClintock, Timothy S.
AU - Xu, Fuqiang
AU - Quintero, Jorge
AU - Gress, Anne M.
AU - Landers, Teresa M.
PY - 1997/6
Y1 - 1997/6
N2 - We have isolated from an American lobster (Homarus americanus) olfactory organ cDNA library a clone, hGα(q), with >80% identity to mammalian and arthrupod Gα(q) sequences. In brain and olfactory organ, hGα(q) mRNA was expressed predominantly in neurons, including virtually all the neuronal cell body clusters of the brain. Gα(q) protein was also expressed broadly, appearing on western blots as a single band of 46 kDa in brain, eyestalk, perelopod, dactyl, tail muscle, olfactory organ, and aesthetasc hairs. These results suggest that hGα(q) plays a role in a wide variety of signal transduction events. Its presence in the olfactory aesthetasc hairs, which are almost pure preparations of the outer dendrites of the olfactory receptor neurons, the expression of a single hGα(q) mRNA species (6 kb) in the olfactory organ, and the localization of hGα(q) mRNA predominantly in the olfactory receptor neurons of the olfactory organ strongly suggest that one function of hGα(q) is to mediate olfactory transduction.
AB - We have isolated from an American lobster (Homarus americanus) olfactory organ cDNA library a clone, hGα(q), with >80% identity to mammalian and arthrupod Gα(q) sequences. In brain and olfactory organ, hGα(q) mRNA was expressed predominantly in neurons, including virtually all the neuronal cell body clusters of the brain. Gα(q) protein was also expressed broadly, appearing on western blots as a single band of 46 kDa in brain, eyestalk, perelopod, dactyl, tail muscle, olfactory organ, and aesthetasc hairs. These results suggest that hGα(q) plays a role in a wide variety of signal transduction events. Its presence in the olfactory aesthetasc hairs, which are almost pure preparations of the outer dendrites of the olfactory receptor neurons, the expression of a single hGα(q) mRNA species (6 kb) in the olfactory organ, and the localization of hGα(q) mRNA predominantly in the olfactory receptor neurons of the olfactory organ strongly suggest that one function of hGα(q) is to mediate olfactory transduction.
KW - Crustaceans
KW - G protein
KW - Lobster
KW - Olfaction
KW - Signal transduction
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U2 - 10.1046/j.1471-4159.1997.68062248.x
DO - 10.1046/j.1471-4159.1997.68062248.x
M3 - Article
C2 - 9166716
AN - SCOPUS:0030929910
SN - 0022-3042
VL - 68
SP - 2248
EP - 2254
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 6
ER -