Abstract
Using the yeast two hybrid system, we isolated a rat cDNA (E3-3) coding for a new protein with no homology to any other protein in the database. E3- 3 is ubiquitously expressed. Variants that most likely arise through alternative splicing encode truncated forms of the protein. Testis is the only tissue that predominantly expresses the longest protein variant. When this variant is tagged with enhanced green fluorescent protein, the protein is located in the nucleus.
| Original language | English |
|---|---|
| Pages (from-to) | 224-230 |
| Number of pages | 7 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1353 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 12 1997 |
Bibliographical note
Funding Information:We would like to thank Claudia Cap for sequencing, J. Chalcroft and R. Schorner for help with artwork. The work is supported by the Max-Planck-Society.
Funding
We would like to thank Claudia Cap for sequencing, J. Chalcroft and R. Schorner for help with artwork. The work is supported by the Max-Planck-Society.
| Funders | Funder number |
|---|---|
| Administrative Headquarters of the Max Planck Society |
Keywords
- Cloning
- Nuclear protein
- Splicing
- Yeast two hybrid system
- cDNA, rat
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics