Molecular cloning of the Escherichia coli K-12 entACGBE genes

Carol L. Pickett, La Donna Hayes, Charles F. Earhart

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


A 7-kb piece of Escherichia coli DNA that contains five genes (entA, C, G, B and E) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini- and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an Mr 63000 polypeptide.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalFEMS Microbiology Letters
Issue number1
StatePublished - Sep 1984

Bibliographical note

Funding Information:
This research was supported by Public Health Service grant AI-17794 from the National Institute of Allergy and Infectious Diseases and by grant F-958 from the R.A. Welch Foundation.


  • Enterochelin
  • iron assimilation
  • promotor
  • restriction mapping

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


Dive into the research topics of 'Molecular cloning of the Escherichia coli K-12 entACGBE genes'. Together they form a unique fingerprint.

Cite this