Molecular cloning of the Escherichia coli K-12 entACGBE genes

Carol L. Pickett; La Donna Hayes; Charles F. Earhart

doi:10.1111/j.1574-6968.1984.tb01248.x

Molecular cloning of the Escherichia coli K-12 entACGBE genes

Carol L. Pickett, La Donna Hayes, Charles F. Earhart

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A 7-kb piece of Escherichia coli DNA that contains five genes (entA, C, G, B and E) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini- and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an M_r 63000 polypeptide.

Original language	English
Pages (from-to)	77-80
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	24
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1984.tb01248.x
State	Published - Sep 1984

Bibliographical note

Funding Information:
This research was supported by Public Health Service grant AI-17794 from the National Institute of Allergy and Infectious Diseases and by grant F-958 from the R.A. Welch Foundation.

Keywords

Enterochelin
iron assimilation
promotor
restriction mapping

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1984.tb01248.x

Cite this

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title = "Molecular cloning of the Escherichia coli K-12 entACGBE genes",

abstract = "A 7-kb piece of Escherichia coli DNA that contains five genes (entA, C, G, B and E) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini- and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an Mr 63000 polypeptide.",

keywords = "Enterochelin, iron assimilation, promotor, restriction mapping",

author = "Pickett, {Carol L.} and Hayes, {La Donna} and Earhart, {Charles F.}",

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AU - Pickett, Carol L.

AU - Hayes, La Donna

AU - Earhart, Charles F.

N1 - Funding Information: This research was supported by Public Health Service grant AI-17794 from the National Institute of Allergy and Infectious Diseases and by grant F-958 from the R.A. Welch Foundation.

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N2 - A 7-kb piece of Escherichia coli DNA that contains five genes (entA, C, G, B and E) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini- and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an Mr 63000 polypeptide.

AB - A 7-kb piece of Escherichia coli DNA that contains five genes (entA, C, G, B and E) required for the biosynthesis of the iron transport molecule enterochelin was isolated. A restriction map was constructed and proteins specified by the E. coli DNA were identified in mini- and maxicell systems. Plasmids containing portions of the entACGBE DNA generated by BAL31 digestion or restriction enzyme treatment were constructed; complementation studies done with these indicated that the five genes constitute an operon. The approximate site of the promoter was determined and the product of entE was tentatively identified as an Mr 63000 polypeptide.

KW - Enterochelin

KW - iron assimilation

KW - promotor

KW - restriction mapping

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Molecular cloning of the Escherichia coli K-12 entACGBE genes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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