Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Yating Liu, Wen Xie, Xin Yang, Litao Guo, Shaoli Wang, Qingjun Wu, Zezhong Yang, Xuguo Zhou, Youjun Zhang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
Issue number2
StatePublished - Apr 15 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.


  • Alternative splicing
  • Bemisia tabaci
  • Haplodiploidy
  • PSI
  • Sex determination

ASJC Scopus subject areas

  • Genetics


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