Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Yating Liu; Wen Xie; Xin Yang; Litao Guo; Shaoli Wang; Qingjun Wu; Zezhong Yang; Xuguo Zhou; Youjun Zhang

doi:10.1016/j.gene.2016.01.005

Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Yating Liu, Wen Xie, Xin Yang, Litao Guo, Shaoli Wang, Qingjun Wu, Zezhong Yang, Xuguo Zhou, Youjun Zhang

Entomology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

Original language	English
Pages (from-to)	104-110
Number of pages	7
Journal	Gene
Volume	580
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2016.01.005
State	Published - Apr 15 2016

Bibliographical note

Funding Information:
This research was supported by the National Natural Science Foundation of China ( 31401747 and 31420103919 ), the Beijing Natural Science Foundation ( 6154034 and 6131002 ), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables .

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

Alternative splicing
Bemisia tabaci
Haplodiploidy
PSI
Sex determination

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2016.01.005

Cite this

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title = "Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties",

abstract = "The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.",

keywords = "Alternative splicing, Bemisia tabaci, Haplodiploidy, PSI, Sex determination",

author = "Yating Liu and Wen Xie and Xin Yang and Litao Guo and Shaoli Wang and Qingjun Wu and Zezhong Yang and Xuguo Zhou and Youjun Zhang",

note = "Funding Information: This research was supported by the National Natural Science Foundation of China ( 31401747 and 31420103919 ), the Beijing Natural Science Foundation ( 6154034 and 6131002 ), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables . Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

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AU - Liu, Yating

AU - Xie, Wen

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AU - Guo, Litao

AU - Wang, Shaoli

AU - Wu, Qingjun

AU - Yang, Zezhong

AU - Zhou, Xuguo

AU - Zhang, Youjun

N1 - Funding Information: This research was supported by the National Natural Science Foundation of China ( 31401747 and 31420103919 ), the Beijing Natural Science Foundation ( 6154034 and 6131002 ), and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables . Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/4/15

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N2 - The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

AB - The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

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Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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