Molecular cloning, sequencing, and expression of equine interleukin-6

C. E. Swiderski; G. Sobol; D. P. Lunn; D. W. Horohov

doi:10.1016/S0165-2427(00)00241-5

Molecular cloning, sequencing, and expression of equine interleukin-6

C. E. Swiderski, G. Sobol, D. P. Lunn, D. W. Horohov

Veterinary Science

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

Original language	English
Pages (from-to)	213-220
Number of pages	8
Journal	Veterinary Immunology and Immunopathology
Volume	77
Issue number	3-4
DOIs	https://doi.org/10.1016/S0165-2427(00)00241-5
State	Published - Dec 29 2000

Bibliographical note

Funding Information:
This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.

Keywords

Equine interleukin-6 (IL-6) cloning expression

ASJC Scopus subject areas

Immunology
General Veterinary

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10.1016/S0165-2427(00)00241-5

Cite this

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title = "Molecular cloning, sequencing, and expression of equine interleukin-6",

abstract = "Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.",

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AU - Sobol, G.

AU - Lunn, D. P.

AU - Horohov, D. W.

N1 - Funding Information: This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.

PY - 2000/12/29

Y1 - 2000/12/29

N2 - Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

AB - Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

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Molecular cloning, sequencing, and expression of equine interleukin-6

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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