Abstract
Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.
| Original language | English |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 77 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Dec 29 2000 |
Bibliographical note
Funding Information:This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.
Funding
This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | |
| Grayson Jockey Club Research Foundation Inc | |
| Louisiana State University |
Keywords
- Equine interleukin-6 (IL-6) cloning expression
ASJC Scopus subject areas
- Immunology
- General Veterinary