Molecular Genetic Manipulation of Sarcocystis neurona

Daniel K. Howe, Michelle Yeargan, Landon Simpson, Sriveny Dangoudoubiyam

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Sarcocystis neurona is a member of the important phylum Apicomplexa and the primary cause of equine protozoal myeloencephalitis (EPM). Moreover, S. neurona is the best-studied species in the genus Sarcocystis, one of the most successful parasite taxa, as virtually all vertebrate animals may be infected by at least one species. Consequently, scientific investigation of S. neurona will aid in the control of EPM and neurologic disease in sea mammals, while also improving our understanding of a prominent branch on the apicomplexan phylogenetic tree. These protocols describe methods that expand the capabilities to study this prominent member of the Apicomplexa.

Original languageEnglish
Pages (from-to)20D.2.1-20D.2.14
JournalCurrent Protocols in Microbiology
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2018

Bibliographical note

Funding Information:
This work was supported by the USDA National Institute of Food and Agriculture (grant no. 2009-65109-05918 and Hatch project accession no. 227792) and by funds from the Amerman Family Equine Research Fund.

Publisher Copyright:
Copyright © 2018 John Wiley & Sons, Inc.

Keywords

  • Apicomplexa
  • CRISPR
  • Sarcocystis neurona
  • transfection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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