Molecular targeting of nuclear transcriptional regulators

Yon Rojanasakul, Q. Luo, J. Ye, D. Faroonsargn, S. Dokka, X. Shi

Research output: Contribution to journalArticlepeer-review

Abstract

Gene regulation by nuclear transcription factors involves intracellular interactions of nuclear transcriptors with specific transport carriers and/or DNA regulatory elements using sequence-specific recognition sites. In this study, we utilized a series of synthetic peptides carrying the nuclear localization sequence of the transcriptor NF-κB p50 and p65 subunits to inhibit NF-κB-mediated gene expression. Gene transfection and gel-shift assays showed that these peptides were effective in inhibiting the nuclear translocation and transcriptional activation of NF-κB. However, their effects required the presence of a cell-permeable peptide which is covalently linked to the inhibitory peptides. These hybrid peptides may be used as novel therapeutic agents in diseases whose etiology is dependent on NF-κB activation.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalACS Symposium Series
Volume752
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Chemistry (all)
  • Chemical Engineering (all)

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