Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

J. Qiu; D. Jin; C. Li; J. Jia; P. Ouyang; W. Tai; B. Wang; D. Li

doi:10.1007/BF02887099

Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

J. Qiu, D. Jin, C. Li, J. Jia, P. Ouyang, W. Tai, B. Wang, D. Li

Research output: Contribution to journal › Article › peer-review

Abstract

RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.

Original language	English
Pages (from-to)	528-532
Number of pages	5
Journal	Chinese Science Bulletin
Volume	45
Issue number	6
DOIs	https://doi.org/10.1007/BF02887099
State	Published - 2000

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 39650001) and Chinese Academy of Sciences Programmes (Grant Nos. KZ95 1-A1-101, KZ95T-02).

Keywords

Bread wheat
RAPD
RFLP
Tripsacum dactyloides
Wide hybridization

ASJC Scopus subject areas

General

Access to Document

10.1007/BF02887099

Cite this

@article{943abf7f1ffe4048af96130d9ebd7cb6,

title = "Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization",

abstract = "RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.",

keywords = "Bread wheat, RAPD, RFLP, Tripsacum dactyloides, Wide hybridization",

author = "J. Qiu and D. Jin and C. Li and J. Jia and P. Ouyang and W. Tai and B. Wang and D. Li",

note = "Funding Information: Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 39650001) and Chinese Academy of Sciences Programmes (Grant Nos. KZ95 1-A1-101, KZ95T-02).",

year = "2000",

doi = "10.1007/BF02887099",

language = "English",

volume = "45",

pages = "528--532",

number = "6",

}

TY - JOUR

T1 - Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

AU - Qiu, J.

AU - Jin, D.

AU - Li, C.

AU - Jia, J.

AU - Ouyang, P.

AU - Tai, W.

AU - Wang, B.

AU - Li, D.

N1 - Funding Information: Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 39650001) and Chinese Academy of Sciences Programmes (Grant Nos. KZ95 1-A1-101, KZ95T-02).

PY - 2000

Y1 - 2000

N2 - RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.

AB - RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.

KW - Bread wheat

KW - RAPD

KW - RFLP

KW - Tripsacum dactyloides

KW - Wide hybridization

UR - http://www.scopus.com/inward/record.url?scp=0034480817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034480817&partnerID=8YFLogxK

U2 - 10.1007/BF02887099

DO - 10.1007/BF02887099

M3 - Article

AN - SCOPUS:0034480817

VL - 45

SP - 528

EP - 532

IS - 6

ER -

Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this