Abstract
RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 528-532 |
| Number of pages | 5 |
| Journal | Chinese Science Bulletin |
| Volume | 45 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2000 |
Bibliographical note
Funding Information:Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 39650001) and Chinese Academy of Sciences Programmes (Grant Nos. KZ95 1-A1-101, KZ95T-02).
Funding
Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 39650001) and Chinese Academy of Sciences Programmes (Grant Nos. KZ95 1-A1-101, KZ95T-02).
| Funders | Funder number |
|---|---|
| Chinese Academy of Sciences Programmes | KZ95T-02, KZ95 1-A1-101 |
| National Natural Science Foundation of China (NSFC) | 39650001 |
Keywords
- Bread wheat
- RAPD
- RFLP
- Tripsacum dactyloides
- Wide hybridization
ASJC Scopus subject areas
- General
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