TY - JOUR
T1 - Monitoring of metabolic responses of intact Haliotis (abalones) under salinity stress by 31P surface probe localized NMR
AU - Higashi, Richard M.
AU - Fan, Teresa W.‐M
AU - Macdonald, Jeffrey M.
PY - 1989/3
Y1 - 1989/3
N2 - Surface probe localized 31P NMR spectroscopy was employed to record the metabolic responses of the foot of intact Haliotis cracherodii and H. rufescens (black and red abalones) under hyper‐ and hypoosmotic stresses. Use of the surface probe allowed spectral localization on the foot of intact abalones, facilitated monitoring of different sizes of animals, and minimized constraints on aquatic chamber design normally imposed by homogeneous‐field probes. Generally, hyperosmotic stress (51‰) elicited more rapid changes of phosphate metabolites than hypoosmotic stress (17‰). As with the well‐studied hypoxic stress in intact mammalian and excised molluscan tissue, both salinity treatments caused drops in the phosphagen and increases in inorganic phosphate levels. However, osmotic stress was distinct from hypoxic stress inthat intracellular pH did not change and nucleotide triphosphate (NTP) concentrations dropped immediately. Although these findings are preliminary, they demonstrate the utility of the surface probe approach for studies of environmental stress in intact marine invertebrates.
AB - Surface probe localized 31P NMR spectroscopy was employed to record the metabolic responses of the foot of intact Haliotis cracherodii and H. rufescens (black and red abalones) under hyper‐ and hypoosmotic stresses. Use of the surface probe allowed spectral localization on the foot of intact abalones, facilitated monitoring of different sizes of animals, and minimized constraints on aquatic chamber design normally imposed by homogeneous‐field probes. Generally, hyperosmotic stress (51‰) elicited more rapid changes of phosphate metabolites than hypoosmotic stress (17‰). As with the well‐studied hypoxic stress in intact mammalian and excised molluscan tissue, both salinity treatments caused drops in the phosphagen and increases in inorganic phosphate levels. However, osmotic stress was distinct from hypoxic stress inthat intracellular pH did not change and nucleotide triphosphate (NTP) concentrations dropped immediately. Although these findings are preliminary, they demonstrate the utility of the surface probe approach for studies of environmental stress in intact marine invertebrates.
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U2 - 10.1002/jez.1402490317
DO - 10.1002/jez.1402490317
M3 - Article
C2 - 2708953
AN - SCOPUS:0024639050
SN - 0022-104X
VL - 249
SP - 350
EP - 356
JO - Journal of Experimental Zoology
JF - Journal of Experimental Zoology
IS - 3
ER -