Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings

T. K. Zagranichnaya; F. A. Brovko; A. O. Shepelyakovskaya; Kh M. Boziev; V. M. Lipkin

Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings

T. K. Zagranichnaya, F. A. Brovko, A. O. Shepelyakovskaya, Kh M. Boziev, V. M. Lipkin

Internal Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.

Original language	English
Pages (from-to)	1-5
Number of pages	5
Journal	Russian Journal of Plant Physiology
Volume	44
Issue number	1
State	Published - Jan 1997

Keywords

Affinity chromatography
Cytokinin-binding proteins
Cytokinins
Monoclonal antibodies
Zea mays

ASJC Scopus subject areas

Plant Science

Cite this

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title = "Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings",

abstract = "Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.",

keywords = "Affinity chromatography, Cytokinin-binding proteins, Cytokinins, Monoclonal antibodies, Zea mays",

author = "Zagranichnaya, {T. K.} and Brovko, {F. A.} and Shepelyakovskaya, {A. O.} and Boziev, {Kh M.} and Lipkin, {V. M.}",

year = "1997",

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T1 - Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings

AU - Zagranichnaya, T. K.

AU - Brovko, F. A.

AU - Shepelyakovskaya, A. O.

AU - Boziev, Kh M.

AU - Lipkin, V. M.

PY - 1997/1

Y1 - 1997/1

N2 - Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.

AB - Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.

KW - Affinity chromatography

KW - Cytokinin-binding proteins

KW - Cytokinins

KW - Monoclonal antibodies

KW - Zea mays

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Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings

Abstract

Keywords

ASJC Scopus subject areas

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