TY - JOUR
T1 - Monoclonal Antibodies to a 70-kD Cytokinin-binding protein from etiolated maize seedlings
AU - Zagranichnaya, T. K.
AU - Brovko, F. A.
AU - Shepelyakovskaya, A. O.
AU - Boziev, Kh M.
AU - Lipkin, V. M.
PY - 1997/1
Y1 - 1997/1
N2 - Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.
AB - Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.
KW - Affinity chromatography
KW - Cytokinin-binding proteins
KW - Cytokinins
KW - Monoclonal antibodies
KW - Zea mays
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M3 - Article
AN - SCOPUS:0031541510
SN - 1021-4437
VL - 44
SP - 1
EP - 5
JO - Russian Journal of Plant Physiology
JF - Russian Journal of Plant Physiology
IS - 1
ER -