Abstract
Monoclonal antibodies to a 70-kD cytokinin-binding protein (CBP-70) from etiolated maize seedlings were obtained. From mouse ascites fluid, seven types of monoclonal antibodies possessing high specificity toward CBP-70 and devoid of cross-reactivity with other soluble proteins from etiolated maize seedlings were isolated and characterized. A protocol for CBP-70 isolation including affinity chromatography on immobilized monoclonal antibodies is developed.
Original language | English |
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Pages (from-to) | 1-5 |
Number of pages | 5 |
Journal | Russian Journal of Plant Physiology |
Volume | 44 |
Issue number | 1 |
State | Published - Jan 1997 |
Keywords
- Affinity chromatography
- Cytokinin-binding proteins
- Cytokinins
- Monoclonal antibodies
- Zea mays
ASJC Scopus subject areas
- Plant Science