Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis

Ren Xu, Li Xin, Yi Fan, Hai Rong Meng, Zai Ping Li, Ren Bao Gan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%-70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G1 arrest and apoptosis in BAE cells.

Original languageEnglish
Pages (from-to)138-142
Number of pages5
JournalActa Biochimica et Biophysica Sinica
Volume34
Issue number2
StatePublished - 2002

Keywords

  • Angiogenesis inhibitor
  • Apoptosis
  • Collagen XV
  • Endostatin
  • Restin

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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