TY - JOUR
T1 - Mouse restin inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis
AU - Xu, Ren
AU - Xin, Li
AU - Fan, Yi
AU - Meng, Hai Rong
AU - Li, Zai Ping
AU - Gan, Ren Bao
PY - 2002
Y1 - 2002
N2 - Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%-70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G1 arrest and apoptosis in BAE cells.
AB - Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%-70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G1 arrest and apoptosis in BAE cells.
KW - Angiogenesis inhibitor
KW - Apoptosis
KW - Collagen XV
KW - Endostatin
KW - Restin
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M3 - Article
C2 - 12007010
AN - SCOPUS:0036034844
SN - 0582-9879
VL - 34
SP - 138
EP - 142
JO - Acta Biochimica et Biophysica Sinica
JF - Acta Biochimica et Biophysica Sinica
IS - 2
ER -