Multienzyme immobilized polymeric membrane reactor for the transformation of a lignin model compound

Rupam Sarma, Md Saiful Islam, Mark P. Running, Dibakar Bhattacharyya

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We have developed an integrated, multienzyme functionalized membrane reactor for bioconversion of a lignin model compound involving enzymatic catalysis. The membrane bioreactors were fabricated through the layer-by-layer assembly approach to immobilize three different enzymes (glucose oxidase, peroxidase and laccase) into pH-responsive membranes. This novel membrane reactor couples the in situ generation of hydrogen peroxide (by glucose oxidase) to oxidative conversion of a lignin model compound, guaiacylglycerol-β-guaiacyl ether (GGE). Preliminary investigation of the efficacy of these functional membranes towards GGE degradation is demonstrated under convective flow mode. Over 90% of the initial feed could be degraded with the multienzyme immobilized membranes at a residence time of approximately 22 s. GGE conversion product analysis revealed the formation of oligomeric oxidation products upon reaction with peroxidase, which may be a potential hazard to membrane bioreactors. These oxidation products could further be degraded by laccase enzymes in the multienzymatic membranes, explaining the potential of multi enzyme membrane reactors. Themultienzyme incorporatedmembrane reactors were active formore than 30 days of storage time at 4 °C. During this time span, repetitive use of the membrane reactor was demonstrated involving 5-6 h of operation time for each cycle. The membrane reactor displayed encouraging performance, losing only 12% of its initial activity after multiple cycles of operation.

Original languageEnglish
Article number463
Issue number4
StatePublished - Apr 2018

Bibliographical note

Publisher Copyright:
© 2018 by the authors.


  • Layer-by-layer assembly
  • Lignin
  • Multienzyme
  • Polymer membrane reactor

ASJC Scopus subject areas

  • General Chemistry
  • Polymers and Plastics


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