Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala

Keyi Fu; Christopher L. Schardl; Daniel Cook; Xuanli Cao; Ning Ling; Chunyu He; Dandan Wu; Longhai Xue; Yanzhong Li; Zunji Shi

doi:10.1021/acs.jafc.3c09049

Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala

Keyi Fu, Christopher L. Schardl, Daniel Cook, Xuanli Cao, Ning Ling, Chunyu He, Dandan Wu, Longhai Xue, Yanzhong Li, Zunji Shi

Plant Pathology

Research output: Contribution to journal › Article › peer-review

Abstract

Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis (A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala (O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephala were compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathris in the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioides and Colletotrichum americae-borealis by increasing the level of oxidative stress. This work suggested that O. ochrocephala containing A. oxytropis could increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.

Original language	English
Pages (from-to)	2397-2409
Number of pages	13
Journal	Journal of Agricultural and Food Chemistry
Volume	72
Issue number	4
DOIs	https://doi.org/10.1021/acs.jafc.3c09049
State	Published - Jan 31 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society.

Funding

This study was financially supported by the Fundamental Research Funds for the Leading Scientist Project of Qinghai Province (2023-NK-147). The authors are grateful to Metware Biotechnology Co., Ltd for their assistance in metabolomics analysis and Biogoethe Biotechnology (Wuhan) Co., Ltd for their contribution in the supply of reagent kits.

Funders	Funder number
Fundamental Research Funds for the Leading Scientist Project of Qinghai Province	2023-NK-147
Wuhan Metware Biotechnology Co., Ltd.

Keywords

Alternaria oxytropis
Oxytropis ochrocephala
high-throughput sequencing of ITS
pseudotargeted metabolomics
swainsonine

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

Access to Document

10.1021/acs.jafc.3c09049

Cite this

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title = "Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala",

abstract = "Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis (A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala (O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephala were compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathris in the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioides and Colletotrichum americae-borealis by increasing the level of oxidative stress. This work suggested that O. ochrocephala containing A. oxytropis could increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.",

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AU - Fu, Keyi

AU - Schardl, Christopher L.

AU - Cook, Daniel

AU - Cao, Xuanli

AU - Ling, Ning

AU - He, Chunyu

AU - Wu, Dandan

AU - Xue, Longhai

AU - Li, Yanzhong

AU - Shi, Zunji

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Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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