Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala

Keyi Fu, Christopher L. Schardl, Daniel Cook, Xuanli Cao, Ning Ling, Chunyu He, Dandan Wu, Longhai Xue, Yanzhong Li, Zunji Shi

Research output: Contribution to journalArticlepeer-review

Abstract

Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis (A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala (O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephala were compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathris in the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioides and Colletotrichum americae-borealis by increasing the level of oxidative stress. This work suggested that O. ochrocephala containing A. oxytropis could increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.

Original languageEnglish
Pages (from-to)2397-2409
Number of pages13
JournalJournal of Agricultural and Food Chemistry
Volume72
Issue number4
DOIs
StatePublished - Jan 31 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society.

Keywords

  • Alternaria oxytropis
  • Oxytropis ochrocephala
  • high-throughput sequencing of ITS
  • pseudotargeted metabolomics
  • swainsonine

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

Fingerprint

Dive into the research topics of 'Multiomics Reveals Mechanisms of Alternaria oxytropis Inhibiting Pathogenic Fungi in Oxytropis ochrocephala'. Together they form a unique fingerprint.

Cite this