Multiple endosymbiont infections and reproductive manipulations in a linyphiid spider population

M. M. Curry, L. V. Paliulis, K. D. Welch, J. D. Harwood, J. A. White

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In many arthropods, maternally inherited endosymbiotic bacteria can increase infection frequency by manipulating host reproduction. Multiple infections of different bacteria in a single host population are common, yet few studies have documented concurrent endosymbiont phenotypes or explored their potential interactions. We hypothesized that spiders might be a particularly useful taxon for investigating endosymbiont interactions, because they are host to a plethora of endosymbiotic bacteria and frequently exhibit multiple infections. We established two matrilines from the same population of the linyphiid spider Mermessus fradeorum and then used antibiotic curing and controlled mating assays to demonstrate that each matriline was subject to a distinct endosymbiotic reproductive manipulation. One matriline was co-infected with Rickettsia and Wolbachia and produced offspring with a radical female bias. Antibiotic treatment eliminated both endosymbionts and restored an even sex ratio to subsequent generations. Chromosomal and fecundity observations suggest a feminization mechanism. In the other matriline, a separate factorial mating assay of cured and infected spiders demonstrated strong cytoplasmic incompatibility (CI) induced by a different strain of Wolbachia. However, males with this Wolbachia induced only mild CI when mated with the Rickettsia–Wolbachia females. In a subsequent survey of a field population of M. fradeorum, we detected these same three endosymbionts infecting 55% of the spiders in almost all possible combinations, with nearly half of the infected spiders exhibiting multiple infection. Our results suggest that a dynamic network of endosymbionts may interact both within multiply infected hosts and within a population subject to multiple strong reproductive manipulations.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalHeredity
Volume115
Issue number2
DOIs
StatePublished - 2015

Bibliographical note

Funding Information:
We thank A Dehnel, Z Helton and E Squires of the University of Kentucky for help with spider rearing and diagnostic PCR. We also thank all reviewers for their constructive comments on previous drafts of this manuscript. This research was supported by the University of Kentucky College of Agriculture and Department of Entomology and USDA National Institute of Food and Agriculture, Hatch/Multistate project KY00856. The information reported in this paper (15-08-016) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.

Publisher Copyright:
© 2015 Macmillan Publishers Limited All rights reserved.

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'Multiple endosymbiont infections and reproductive manipulations in a linyphiid spider population'. Together they form a unique fingerprint.

Cite this