TY - JOUR
T1 - Multiple forms of endocannabinoid and endovanilloid signaling regulate the tonic control of GABA release
AU - Lee, Sang Hun
AU - Ledri, Marco
AU - Tóth, Blanka
AU - Marchionni, Ivan
AU - Henstridge, Christopher M.
AU - Dudok, Barna
AU - Kenesei, Kata
AU - Barna, László
AU - Szabó, Szilárd I.
AU - Renkecz, Tibor
AU - Oberoi, Michelle
AU - Watanabe, Masahiko
AU - Limoli, Charles L.
AU - Horvai, George
AU - Soltesz, Ivan
AU - Katona, István
N1 - Publisher Copyright:
© 2015 Lee et al.
PY - 2015/7/8
Y1 - 2015/7/8
N2 - PersistentCB1cannabinoidreceptoractivitylimitsneurotransmitterreleaseatvarioussynapsesthroughoutthebrain.However,itisnotfullyunderstood how constitutively active CB1 receptors, tonic endocannabinoid signaling, and its regulation by multiple serine hydrolases contribute to the synapsespecific calibration of neurotransmitter release probability. To address this question at perisomatic and dendritic GABAergic synapses in the mouse hippocampus,weusedacombinationof paired whole-cellpatch-clamprecording, liquidchromatography/tandemmassspectrometry, stochastic optical reconstruction microscopy super-resolution imaging, and immunogold electron microscopy. Unexpectedly, application of the CB1 antagonist and inverseagonistAM251[N-1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-1-piperidinyl-1H-pyrazole-3-carboxamide],butnottheneutralantagonist NESS0327 [8-chloro-1-(2,4-dichlorophenyl)-N-piperidin-1-yl-5,6-dihydro-4H-benzo[2,3]cyclohepta[2,4-b]pyrazole-3-carboxamine], significantly increased synaptic transmission between CB1-positive perisomatic interneurons and CA1 pyramidal neurons. JZL184 (4-nitrophenyl 4-[bis(1,3-benzodioxol-5-yl)(hydroxy)methyl]piperidine-1-carboxylate), a selective inhibitor of monoacylglycerol lipase (MGL), the presynaptic degrading enzyme of the endocannabinoid 2-arachidonoylglycerol (2-AG), elicited a robust increase in 2-AG levels and concomitantly decreased GABAergic transmission. In contrast, inhibition of fatty acid amide hydrolase (FAAH) by PF3845 (N-pyridin-3-yl-4-[[3-[5- (trifluoromethyl)pyridin-2-yl]oxyphenyl]methyl]piperidine-1-carboxamide) elevated endocannabinoid/endovanilloid anandamide levels but did not change GABAergic synaptic activity. However, FAAH inhibitors attenuated tonic 2-AG increase and also decreased its synaptic effects. This antagonistic interaction required the activation of the transient receptor potential vanilloid receptor TRPV1, which was concentrated on postsynaptic intracellular membrane cisternae at perisomatic GABAergic symmetrical synapses. Interestingly, neither AM251, JZL184, nor PF3845 affected CB1-positive dendritic interneuron synapses. Together, these findings are consistent with the possibility that constitutively activeCB1 receptors substantially influence perisomaticGABArelease probability and indicate that the synaptic effects of tonic 2-AG release are tightly controlled by presynapticMGLactivity and also by postsynaptic endovanilloid signaling and FAAH activity.
AB - PersistentCB1cannabinoidreceptoractivitylimitsneurotransmitterreleaseatvarioussynapsesthroughoutthebrain.However,itisnotfullyunderstood how constitutively active CB1 receptors, tonic endocannabinoid signaling, and its regulation by multiple serine hydrolases contribute to the synapsespecific calibration of neurotransmitter release probability. To address this question at perisomatic and dendritic GABAergic synapses in the mouse hippocampus,weusedacombinationof paired whole-cellpatch-clamprecording, liquidchromatography/tandemmassspectrometry, stochastic optical reconstruction microscopy super-resolution imaging, and immunogold electron microscopy. Unexpectedly, application of the CB1 antagonist and inverseagonistAM251[N-1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-1-piperidinyl-1H-pyrazole-3-carboxamide],butnottheneutralantagonist NESS0327 [8-chloro-1-(2,4-dichlorophenyl)-N-piperidin-1-yl-5,6-dihydro-4H-benzo[2,3]cyclohepta[2,4-b]pyrazole-3-carboxamine], significantly increased synaptic transmission between CB1-positive perisomatic interneurons and CA1 pyramidal neurons. JZL184 (4-nitrophenyl 4-[bis(1,3-benzodioxol-5-yl)(hydroxy)methyl]piperidine-1-carboxylate), a selective inhibitor of monoacylglycerol lipase (MGL), the presynaptic degrading enzyme of the endocannabinoid 2-arachidonoylglycerol (2-AG), elicited a robust increase in 2-AG levels and concomitantly decreased GABAergic transmission. In contrast, inhibition of fatty acid amide hydrolase (FAAH) by PF3845 (N-pyridin-3-yl-4-[[3-[5- (trifluoromethyl)pyridin-2-yl]oxyphenyl]methyl]piperidine-1-carboxamide) elevated endocannabinoid/endovanilloid anandamide levels but did not change GABAergic synaptic activity. However, FAAH inhibitors attenuated tonic 2-AG increase and also decreased its synaptic effects. This antagonistic interaction required the activation of the transient receptor potential vanilloid receptor TRPV1, which was concentrated on postsynaptic intracellular membrane cisternae at perisomatic GABAergic symmetrical synapses. Interestingly, neither AM251, JZL184, nor PF3845 affected CB1-positive dendritic interneuron synapses. Together, these findings are consistent with the possibility that constitutively activeCB1 receptors substantially influence perisomaticGABArelease probability and indicate that the synaptic effects of tonic 2-AG release are tightly controlled by presynapticMGLactivity and also by postsynaptic endovanilloid signaling and FAAH activity.
KW - 2-arachidonoylglycerol
KW - Endocannabinod
KW - GABA
KW - Hippocampus
KW - Interneuron
KW - TRPV1
UR - http://www.scopus.com/inward/record.url?scp=84936883088&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84936883088&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.4112-14.2015
DO - 10.1523/JNEUROSCI.4112-14.2015
M3 - Article
C2 - 26157003
AN - SCOPUS:84936883088
SN - 0270-6474
VL - 35
SP - 10039
EP - 10057
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 27
ER -