Multiple shoot formation from cotyledonary node segments of Eastern redbud

K. Distabanjong, R. L. Geneve

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


A procedure for multiple shoot formation from cotyledonary node explants of Eastern redbud (Cercis canadensis L.) cultured on DKW medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. Explants on medium with TDZ in combination with BA produced higher numbers of shoots than with either cytokinin alone. The highest number of shoots (7.8 to 9.8 shoots per explant) was obtained when explants from 4 to 10 day-old seedlings were treated with a combination of 10 or 15 μM BA and 0.5 or 1.0 μM TDZ for 20 days before being transferred to the same medium without TDZ. The number of shoots formed was increased from 5.8 to 7.2 shoots per explant by cutting through the cotyledonary node prior to culture. Histological studies indicated that the shoots were formed from actively dividing cells located at the axillary bud region. Shoots formed roots in half strength woody plant medium (WPM) supplemented with 10 to 200 μM indole-3-butyric acid (IBA) cultured for 15 days prior to transfer to greenhouse medium.

Original languageEnglish
Pages (from-to)247-254
Number of pages8
JournalPlant Cell, Tissue and Organ Culture
Issue number3
StatePublished - Oct 1997


  • BA
  • Cercis canadensis
  • IBA
  • leguminous tree
  • organogenesis
  • root formation
  • TDZ

ASJC Scopus subject areas

  • Horticulture


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