Murine norovirus infection does not cause major disruptions in the murine intestinal microbiota

Adam M. Nelson; Michael D. Elftman; Amelia K. Pinto; Megan Baldridge; Patrick Hooper; Justin Kuczynski; Joseph F. Petrosino; Vincent B. Young; Christiane E. Wobus

doi:10.1186/2049-2618-1-7

Murine norovirus infection does not cause major disruptions in the murine intestinal microbiota

Adam M. Nelson, Michael D. Elftman, Amelia K. Pinto, Megan Baldridge, Patrick Hooper, Justin Kuczynski, Joseph F. Petrosino, Vincent B. Young, Christiane E. Wobus

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Background: Murine norovirus (MNV) is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease (IBD). Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice. Results: The composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL/6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection. Conclusions: This is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL/6 mice.

Original language	English
Article number	7
Journal	Microbiome
Volume	1
Issue number	1
DOIs	https://doi.org/10.1186/2049-2618-1-7
State	Published - Feb 18 2013

Bibliographical note

Funding Information:
This work was supported by Award no. 5 U01 AI075396 from the National Institute of Allergy and Infectious Diseases (NIAID) to VBY, the University of Michigan T32 Pulmonary training grant to AMN, by a University of Michigan Genetics and Genomics Pilot Feasibility Grant to CEW. MDE was supported by the University of Michigan Experimental Immunology Training Grant (2 T32 AI007413-18). AKP was supported by NIH grants AI084887, and AI054483 to H W Virgin. We thank Cheryl Perkins and Dr Kenneth Henderson at Charles River Laboratories for assistance with MNV PCR and Dr Larissa Thackray for critical reading of the manuscript.

Funding

This work was supported by Award no. 5 U01 AI075396 from the National Institute of Allergy and Infectious Diseases (NIAID) to VBY, the University of Michigan T32 Pulmonary training grant to AMN, by a University of Michigan Genetics and Genomics Pilot Feasibility Grant to CEW. MDE was supported by the University of Michigan Experimental Immunology Training Grant (2 T32 AI007413-18). AKP was supported by NIH grants AI084887, and AI054483 to H W Virgin. We thank Cheryl Perkins and Dr Kenneth Henderson at Charles River Laboratories for assistance with MNV PCR and Dr Larissa Thackray for critical reading of the manuscript.

Funders	Funder number
National Institute of Allergy and Infectious Diseases
National Institutes of Health (NIH)	AI054483, AI084887
National Institute of Allergy and Infectious Diseases
University of Michigan Hospital	2 T32 AI007413-18

Keywords

Microbiome
Murine norovirus
Pyrosequencing

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

Access to Document

10.1186/2049-2618-1-7

Cite this

@article{4993f5d96d3a4e84a313e8eabf0fab84,

title = "Murine norovirus infection does not cause major disruptions in the murine intestinal microbiota",

abstract = "Background: Murine norovirus (MNV) is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease (IBD). Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice. Results: The composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL/6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection. Conclusions: This is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL/6 mice.",

keywords = "Microbiome, Murine norovirus, Pyrosequencing",

author = "Nelson, {Adam M.} and Elftman, {Michael D.} and Pinto, {Amelia K.} and Megan Baldridge and Patrick Hooper and Justin Kuczynski and Petrosino, {Joseph F.} and Young, {Vincent B.} and Wobus, {Christiane E.}",

note = "Funding Information: This work was supported by Award no. 5 U01 AI075396 from the National Institute of Allergy and Infectious Diseases (NIAID) to VBY, the University of Michigan T32 Pulmonary training grant to AMN, by a University of Michigan Genetics and Genomics Pilot Feasibility Grant to CEW. MDE was supported by the University of Michigan Experimental Immunology Training Grant (2 T32 AI007413-18). AKP was supported by NIH grants AI084887, and AI054483 to H W Virgin. We thank Cheryl Perkins and Dr Kenneth Henderson at Charles River Laboratories for assistance with MNV PCR and Dr Larissa Thackray for critical reading of the manuscript.",

year = "2013",

month = feb,

day = "18",

doi = "10.1186/2049-2618-1-7",

language = "English",

volume = "1",

journal = "Microbiome",

issn = "2049-2618",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - Murine norovirus infection does not cause major disruptions in the murine intestinal microbiota

AU - Nelson, Adam M.

AU - Elftman, Michael D.

AU - Pinto, Amelia K.

AU - Baldridge, Megan

AU - Hooper, Patrick

AU - Kuczynski, Justin

AU - Petrosino, Joseph F.

AU - Young, Vincent B.

AU - Wobus, Christiane E.

N1 - Funding Information: This work was supported by Award no. 5 U01 AI075396 from the National Institute of Allergy and Infectious Diseases (NIAID) to VBY, the University of Michigan T32 Pulmonary training grant to AMN, by a University of Michigan Genetics and Genomics Pilot Feasibility Grant to CEW. MDE was supported by the University of Michigan Experimental Immunology Training Grant (2 T32 AI007413-18). AKP was supported by NIH grants AI084887, and AI054483 to H W Virgin. We thank Cheryl Perkins and Dr Kenneth Henderson at Charles River Laboratories for assistance with MNV PCR and Dr Larissa Thackray for critical reading of the manuscript.

PY - 2013/2/18

Y1 - 2013/2/18

N2 - Background: Murine norovirus (MNV) is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease (IBD). Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice. Results: The composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL/6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection. Conclusions: This is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL/6 mice.

AB - Background: Murine norovirus (MNV) is the most common gastrointestinal pathogen of research mice and can alter research outcomes in biomedical mouse models of inflammatory bowel disease (IBD). Despite indications that an altered microbiota is a risk factor for IBD, the response of the murine intestinal microbiota to MNV infection has not been examined. Microbiota disruption caused by MNV infection could introduce the confounding effects observed in research experiments. Therefore, this study investigated the effects of MNV infection on the intestinal microbiota of wild-type mice. Results: The composition of the intestinal microbiota was assessed over time in both outbred Swiss Webster and inbred C57BL/6 mice following MNV infection. Mice were infected with both persistent and non-persistent MNV strains and tissue-associated or fecal-associated microbiota was analyzed by 16S rRNA-encoding gene pyrosequencing. Analysis of intestinal bacterial communities in infected mice at the phylum and family level showed no major differences to uninfected controls, both in tissue-associated samples and feces, and also over time following infection, demonstrating that the intestinal microbiota of wild-type mice is highly resistant to disruption following MNV infection. Conclusions: This is the first study to describe the intestinal microbiota following MNV infection and demonstrates that acute or persistent MNV infection is not associated with major disruptions of microbial communities in Swiss Webster and C57BL/6 mice.

KW - Microbiome

KW - Murine norovirus

KW - Pyrosequencing

UR - http://www.scopus.com/inward/record.url?scp=84901331051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901331051&partnerID=8YFLogxK

U2 - 10.1186/2049-2618-1-7

DO - 10.1186/2049-2618-1-7

M3 - Article

AN - SCOPUS:84901331051

SN - 2049-2618

VL - 1

JO - Microbiome

JF - Microbiome

IS - 1

M1 - 7

ER -

Murine norovirus infection does not cause major disruptions in the murine intestinal microbiota

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this