Mutations of genes including DNMT3A detected by next-generation sequencing in thyroid cancer

Ling Chuan Guo; Wei Dong Zhu; Xiang Yuan Ma; Hao Ni; En Jian Zhong; Yang W. Shao; Jie Yu; Dong Mei Gu; Shun Dong Ji; Hao Dong Xu; Cheng Ji; Jin Ming Yang; Yi Zhang

doi:10.1080/15384047.2018.1523856

Mutations of genes including DNMT3A detected by next-generation sequencing in thyroid cancer

Ling Chuan Guo, Wei Dong Zhu, Xiang Yuan Ma, Hao Ni, En Jian Zhong, Yang W. Shao, Jie Yu, Dong Mei Gu, Shun Dong Ji, Hao Dong Xu, Cheng Ji, Jin Ming Yang, Yi Zhang

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

More than 90% of thyroid cancer belongs to the papillary and follicular thyroid carcinomas based on pathological subtypes. Papillary and follicular thyroid carcinoma are generally associated with a good prognosis. In contrast, other pathological subtypes such as poorly-differentiated and anaplastic thyroid carcinoma (PDTC and ATC) have a poor clinical outcome with a short life expectancy. To identify the genetic variations and biomarkers that may potentially distinguish the aggressive form of thyroid cancer, we performed a retrospective analysis of the formalin-fixed paraffin-embedded tumor samples from 50 patients who mainly displayed aggressive thyroid cancer using next-generation sequencing of 416 solid tumor-related genes. We adopted extensive bioinformatic analysis to vigorously remove germline single-nucleotide polymorphism and systematic sequencing errors, and report here that mutation in DNMT3A gene was significantly enriched in patients with PDTC or ATC.

Original language	English
Pages (from-to)	240-246
Number of pages	7
Journal	Cancer Biology and Therapy
Volume	20
Issue number	3
DOIs	https://doi.org/10.1080/15384047.2018.1523856
State	Published - Mar 4 2019

Bibliographical note

Publisher Copyright:
© 2018, © 2018 Taylor & Francis Group, LLC.

Funding

This study was funded by grants from National Natural Sciences Foundation of China (81473240, 81773749) to Yi Zhang; National Natural Sciences Foundation of China (81472191) to Shundong Ji; Natural Science Foundation of Jiangsu Province of China (BK20151209) to Cheng Ji; and sponsored by Qing Lan Project to Yi Zhang.

Funders	Funder number
National Natural Sciences Foundation of China
National Natural Science Foundation of China (NSFC)	81473240, 81472191, 81773749
Natural Science Foundation of Jiangsu Province	BK20151209
Qinglan Project of Jiangsu Province of China

Keywords

gene mutation
next-generation sequencing
thyroid carcinoma

ASJC Scopus subject areas

Molecular Medicine
Oncology
Pharmacology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/15384047.2018.1523856

Cite this

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title = "Mutations of genes including DNMT3A detected by next-generation sequencing in thyroid cancer",

abstract = "More than 90% of thyroid cancer belongs to the papillary and follicular thyroid carcinomas based on pathological subtypes. Papillary and follicular thyroid carcinoma are generally associated with a good prognosis. In contrast, other pathological subtypes such as poorly-differentiated and anaplastic thyroid carcinoma (PDTC and ATC) have a poor clinical outcome with a short life expectancy. To identify the genetic variations and biomarkers that may potentially distinguish the aggressive form of thyroid cancer, we performed a retrospective analysis of the formalin-fixed paraffin-embedded tumor samples from 50 patients who mainly displayed aggressive thyroid cancer using next-generation sequencing of 416 solid tumor-related genes. We adopted extensive bioinformatic analysis to vigorously remove germline single-nucleotide polymorphism and systematic sequencing errors, and report here that mutation in DNMT3A gene was significantly enriched in patients with PDTC or ATC.",

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doi = "10.1080/15384047.2018.1523856",

language = "English",

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AU - Guo, Ling Chuan

AU - Zhu, Wei Dong

AU - Ma, Xiang Yuan

AU - Ni, Hao

AU - Zhong, En Jian

AU - Shao, Yang W.

AU - Yu, Jie

AU - Gu, Dong Mei

AU - Ji, Shun Dong

AU - Xu, Hao Dong

AU - Ji, Cheng

AU - Yang, Jin Ming

AU - Zhang, Yi

PY - 2019/3/4

Y1 - 2019/3/4

N2 - More than 90% of thyroid cancer belongs to the papillary and follicular thyroid carcinomas based on pathological subtypes. Papillary and follicular thyroid carcinoma are generally associated with a good prognosis. In contrast, other pathological subtypes such as poorly-differentiated and anaplastic thyroid carcinoma (PDTC and ATC) have a poor clinical outcome with a short life expectancy. To identify the genetic variations and biomarkers that may potentially distinguish the aggressive form of thyroid cancer, we performed a retrospective analysis of the formalin-fixed paraffin-embedded tumor samples from 50 patients who mainly displayed aggressive thyroid cancer using next-generation sequencing of 416 solid tumor-related genes. We adopted extensive bioinformatic analysis to vigorously remove germline single-nucleotide polymorphism and systematic sequencing errors, and report here that mutation in DNMT3A gene was significantly enriched in patients with PDTC or ATC.

AB - More than 90% of thyroid cancer belongs to the papillary and follicular thyroid carcinomas based on pathological subtypes. Papillary and follicular thyroid carcinoma are generally associated with a good prognosis. In contrast, other pathological subtypes such as poorly-differentiated and anaplastic thyroid carcinoma (PDTC and ATC) have a poor clinical outcome with a short life expectancy. To identify the genetic variations and biomarkers that may potentially distinguish the aggressive form of thyroid cancer, we performed a retrospective analysis of the formalin-fixed paraffin-embedded tumor samples from 50 patients who mainly displayed aggressive thyroid cancer using next-generation sequencing of 416 solid tumor-related genes. We adopted extensive bioinformatic analysis to vigorously remove germline single-nucleotide polymorphism and systematic sequencing errors, and report here that mutation in DNMT3A gene was significantly enriched in patients with PDTC or ATC.

KW - gene mutation

KW - next-generation sequencing

KW - thyroid carcinoma

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Mutations of genes including DNMT3A detected by next-generation sequencing in thyroid cancer

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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