Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion

Yuan Wen, Davis A. Englund, Bailey D. Peck, Kevin A. Murach, John J. McCarthy, Charlotte A. Peterson

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle. After 4 weeks of exercise training, snRNA-seq reveals that resident muscle stem cells, or satellite cells, are activated with acute exercise but demonstrate limited lineage progression while contributing to muscle adaptation. In the absence of satellite cells, a portion of nuclei demonstrates divergent transcriptional dynamics associated with mixed-fate identities compared with satellite cell replete muscles. These data provide a compendium of information about how satellite cells influence myonuclear transcription in response to exercise.

Original languageEnglish
Article number102838
JournaliScience
Volume24
Issue number8
DOIs
StatePublished - Aug 20 2021

Bibliographical note

Publisher Copyright:
© 2021 The Authors

Keywords

  • Biological sciences
  • Stem cells research
  • Transcriptomics

ASJC Scopus subject areas

  • General

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