N-Methyl-D-aspartate receptor subunit expression and phosphorylation following excitotoxic spinal cord injury in rats

Robert M. Caudle; Federico M. Perez; Christopher King; Chen Guang Yu; Robert P. Yezierski

doi:10.1016/S0304-3940(03)00700-6

N-Methyl-D-aspartate receptor subunit expression and phosphorylation following excitotoxic spinal cord injury in rats

Robert M. Caudle, Federico M. Perez, Christopher King, Chen Guang Yu, Robert P. Yezierski

Neuroscience

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The role of NMDA receptor expression and post-translational modification in the pathological and behavioral consequences of injury were examined in rats receiving spinal injections of quisqualate. Spinal cords were removed 3 days following the development of excessive grooming behavior or, if the spontaneous pain-like behavior was not observed, 13 days following injections. Western blots from the spinal tissue demonstrated that non-grooming animals had elevated protein levels of the NR1 subunit of the NMDA receptor. These subunits did not demonstrate an enhanced level of phosphorylation. NR1 protein in grooming rats was not elevated, but there was a significant increase in NR1 serine phosphorylation. These findings suggest that excitotoxic lesions of the spinal cord induce both NR1 expression and NR1 serine phosphorylation. However, the injury-induced excessive grooming behavior is only associated with phosphorylation of the NR1 subunit.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Neuroscience Letters
Volume	349
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(03)00700-6
State	Published - Sep 25 2003

Bibliographical note

Funding Information:
This work was supported by NIH grants NS40096 (RPY) and DA13166 (RMC), and the Brain and Spinal Cord Injury Rehabilitation Trust Fund (RMC).

Funding

This work was supported by NIH grants NS40096 (RPY) and DA13166 (RMC), and the Brain and Spinal Cord Injury Rehabilitation Trust Fund (RMC).

Funders	Funder number
RMC Research Corporation
National Institutes of Health (NIH)	DA13166
National Institutes of Health (NIH)
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council	R01NS040096
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council
CatWalk Spinal Cord Injury Research Trust

Keywords

Excitotoxicity
N-Methyl-D-aspartate receptors
Phosphorylation
Protein expression
Quisqualate
Spinal cord injury

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0304-3940(03)00700-6

Cite this

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title = "N-Methyl-D-aspartate receptor subunit expression and phosphorylation following excitotoxic spinal cord injury in rats",

abstract = "The role of NMDA receptor expression and post-translational modification in the pathological and behavioral consequences of injury were examined in rats receiving spinal injections of quisqualate. Spinal cords were removed 3 days following the development of excessive grooming behavior or, if the spontaneous pain-like behavior was not observed, 13 days following injections. Western blots from the spinal tissue demonstrated that non-grooming animals had elevated protein levels of the NR1 subunit of the NMDA receptor. These subunits did not demonstrate an enhanced level of phosphorylation. NR1 protein in grooming rats was not elevated, but there was a significant increase in NR1 serine phosphorylation. These findings suggest that excitotoxic lesions of the spinal cord induce both NR1 expression and NR1 serine phosphorylation. However, the injury-induced excessive grooming behavior is only associated with phosphorylation of the NR1 subunit.",

keywords = "Excitotoxicity, N-Methyl-D-aspartate receptors, Phosphorylation, Protein expression, Quisqualate, Spinal cord injury",

author = "Caudle, {Robert M.} and Perez, {Federico M.} and Christopher King and Yu, {Chen Guang} and Yezierski, {Robert P.}",

note = "Funding Information: This work was supported by NIH grants NS40096 (RPY) and DA13166 (RMC), and the Brain and Spinal Cord Injury Rehabilitation Trust Fund (RMC).",

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AU - Caudle, Robert M.

AU - Perez, Federico M.

AU - King, Christopher

AU - Yu, Chen Guang

AU - Yezierski, Robert P.

N1 - Funding Information: This work was supported by NIH grants NS40096 (RPY) and DA13166 (RMC), and the Brain and Spinal Cord Injury Rehabilitation Trust Fund (RMC).

PY - 2003/9/25

Y1 - 2003/9/25

N2 - The role of NMDA receptor expression and post-translational modification in the pathological and behavioral consequences of injury were examined in rats receiving spinal injections of quisqualate. Spinal cords were removed 3 days following the development of excessive grooming behavior or, if the spontaneous pain-like behavior was not observed, 13 days following injections. Western blots from the spinal tissue demonstrated that non-grooming animals had elevated protein levels of the NR1 subunit of the NMDA receptor. These subunits did not demonstrate an enhanced level of phosphorylation. NR1 protein in grooming rats was not elevated, but there was a significant increase in NR1 serine phosphorylation. These findings suggest that excitotoxic lesions of the spinal cord induce both NR1 expression and NR1 serine phosphorylation. However, the injury-induced excessive grooming behavior is only associated with phosphorylation of the NR1 subunit.

AB - The role of NMDA receptor expression and post-translational modification in the pathological and behavioral consequences of injury were examined in rats receiving spinal injections of quisqualate. Spinal cords were removed 3 days following the development of excessive grooming behavior or, if the spontaneous pain-like behavior was not observed, 13 days following injections. Western blots from the spinal tissue demonstrated that non-grooming animals had elevated protein levels of the NR1 subunit of the NMDA receptor. These subunits did not demonstrate an enhanced level of phosphorylation. NR1 protein in grooming rats was not elevated, but there was a significant increase in NR1 serine phosphorylation. These findings suggest that excitotoxic lesions of the spinal cord induce both NR1 expression and NR1 serine phosphorylation. However, the injury-induced excessive grooming behavior is only associated with phosphorylation of the NR1 subunit.

KW - Excitotoxicity

KW - N-Methyl-D-aspartate receptors

KW - Phosphorylation

KW - Protein expression

KW - Quisqualate

KW - Spinal cord injury

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N-Methyl-D-aspartate receptor subunit expression and phosphorylation following excitotoxic spinal cord injury in rats

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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