Lack of oxygen (hypoxia and anoxia) is detrimental to cell function and survival and underlies many disease conditions. Hence, metazoans have evolved mechanisms to adapt to low oxygen. One such mechanism, metabolic suppression, decreases the cellular demand for oxygen by downregulating ATP-demanding processes. However, the molecular mechanisms underlying this adaptation are poorly understood. Here, we report on the role of ndrg1a in hypoxia adaptation of the anoxia-tolerant zebrafish embryo. ndrg1a is expressed in the kidney and ionocytes, cell types that use large amounts of ATP to maintain ion homeostasis. ndrg1a mutants are viable and develop normally when raised under normal oxygen. However, their survival and kidney function is reduced relative to WT embryos following exposure to prolonged anoxia. We further demonstrate that Ndrg1a binds to the energy-demanding sodium-potassium ATPase (NKA) pump under anoxia and is required for its degradation, which may preserve ATP in the kidney and ionocytes and contribute to energy homeostasis. Lastly, we show that sodium azide treatment, which increases lactate levels under normoxia, is sufficient to trigger NKA degradation in an Ndrg1a-dependent manner. These findings support a model whereby Ndrg1a is essential for hypoxia adaptation and functions downstream of lactate signaling to induce NKA degradation, a process known to conserve cellular energy.
|State||Published - Oct 2022|
Bibliographical noteFunding Information:
We acknowledge M Van Doren, D Eisenmann, S Miller, P Robinson and B Weinstein for their helpful comments on our manuscript. We also acknowledge T de Carvalho and S Larson for help with imaging during the COVID19 pandemic. In addition, access to ITC facilities in the HHMI lab at UMBC (Michael F Summers) is gratefully acknowledged. This project was supported by funding from the Department of Defense (W81XWH-16-1-0466) and the National Institute of Health/NICHD (R21HD089476) to R Brewster and Y.-S Lee. We thank T Addo for contributing to the analysis of ndrg1a function. A Gabel, B Canales and A Osei-Ntansah were supported by a National Institute of Health /NIGMS MARC U*STAR (T34 HHS 00001) National Research Service Award to UMBC. S Viswanathan was supported in part by a grant to UMBC from the Howard Hughes Medical Institute through the HHMI Adaptation Project. Polina Kassir was supported by Experimental Learning Award through the Sondheim Public Affairs Scholars Program.
© Park et al.
ASJC Scopus subject areas
- Neuroscience (all)
- Biochemistry, Genetics and Molecular Biology (all)
- Immunology and Microbiology (all)