NADPH-dependent flavoenzymes catalyze one electron reduction of metal ions and molecular oxygen and generate hydroxyl radicals

Xianglin Shi, N. S. Dalal

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

This study reports a new property of the important NAD(P)H-dependent flavoenzymes, glutathione reductase, lipoyl dehydrogenase and ferredoxin-NADP+ oxidoreductase, that can catalyze a one electron reduction of metal ions such as chromium(VI) and vanadium(V). During the enzymatic reduction process, molecular oxygen is reduced to H2O2, which reacts with the reduced metal complexes to generate hydroxyl radicals. Since the hydroxyl radicals have been suggested to play an important role in Cr(VI) toxicity, this study provides a basis for a recent observation that Cr(VI) mutagenesis is strongly oxygen dependent. These results also point to an enzymatic pathway for the metabolism of some metal ions and concomitant generation of hydroxyl radicals.

Original languageEnglish
Pages (from-to)189-191
Number of pages3
JournalFEBS Letters
Volume276
Issue number1-2
DOIs
StatePublished - Dec 10 1990

Keywords

  • ESR
  • Flavoenzyme
  • Hydroxyl radical
  • Reduction of metal ions and molecular oxygen
  • Spin trapping

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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